ArticleJournal of virology2001
Rescue of recombinant Thogoto virus from cloned cDNA.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Unlocking influenza B: exploring molecular biology and reverse genetics for epidemic control and vaccine innovation.Virology journal · 2024Review
- Eight years' advances on Bourbon virus, a tick-born Thogotovirus of theZoonoses (Shannon, Ireland) · 2022Article
- Influenza Reverse Genetics-Historical Perspective.Cold Spring Harbor perspectives in medicine · 2021Review
- Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity.PLoS pathogens · 2018Article
- Many ways to make an influenza virus--review of influenza virus reverse genetics methods.Influenza and other respiratory viruses · 2013Review
- The cytoplasmic location of chicken mx is not the determining factor for its lack of antiviral activity.PloS one · 2010Article
- The interferon antagonist ML protein of thogoto virus targets general transcription factor IIB.Journal of virology · 2008Article
- Rescue of influenza C virus from recombinant DNA.Journal of virology · 2007Article
- A mutation on influenza C virus M1 protein affects virion morphology by altering the membrane affinity of the protein.Journal of virology · 2007Article
- RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication.Journal of virology · 2006Article
- Thogoto virus lacking interferon-antagonistic protein ML is strongly attenuated in newborn Mx1-positive but not Mx1-negative mice.Journal of virology · 2004Article
- Novel gene product of Thogoto virus segment 6 codes for an interferon antagonist.Journal of virology · 2003Article
- Rescue of influenza B virus from eight plasmids.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thogoto virus (THOV) is a tick-transmitted orthomyxovirus with a genome consisting of six negative-stranded RNA segments. To rescue a recombinant THOV, the viral structural proteins were produced from expression plasmids by means of a vaccinia virus expressing the T7 RNA polymerase. Genomic virus RNAs (vRNAs) were generated from plasmids under the control of the RNA polymerase I promoter. Using this system, we could efficiently recover recombinant THOV following transfection of 12 plasmids into 293T cells. To verify the recombinant nature of the rescued virus, specific genetic tags were introduced into two vRNA segments. The availability of this efficient reverse genetics system will allow us to address hitherto-unanswered questions regarding the biology of THOV by manipulating viral genes in the context of infectious virus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.