ArticleJournal of virology2001
A cucumber mosaic virus (CMV) RNA 1 transgene mediates suppression of the homologous viral RNA 1 constitutively and prevents CMV entry into the phloem.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 36 citations in OpenAlex.
- A conserved RNA structure is essential for a satellite RNA-mediated inhibition of helper virus accumulation.Nucleic acids research · 2019Article
- Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants.Scientific reports · 2018Article
- HR-Mediated Defense Response is Overcome at High Temperatures inThe plant pathology journal · 2018Article
- cmv1 is a gate for Cucumber mosaic virus transport from bundle sheath cells to phloem in melon.Molecular plant pathology · 2016Article
- Transgenic tobacco lines expressing defective CMV replicase-derived dsRNA are resistant to CMV-O and CMV-Y.Molecular biotechnology · 2014Article
- Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded RNA modulates plant defence against CMV strains O and Y in potato.Transgenic research · 2013Article
- Transgenic resistance to Bamboo mosaic virus by expression of interfering satellite RNA.Molecular plant pathology · 2013Article
- Suppressors of RNAi from plant viruses are subject to episodic positive selection.Proceedings. Biological sciences · 2013Article
- Helicase domain encoded by Cucumber mosaic virus RNA1 determines systemic infection of Cmr1 in pepper.PloS one · 2012Article
- Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance to Cucumber mosaic virus (CMV) in tobacco lines transformed with CMV RNA 1 is characterized by reduced virus accumulation in the inoculated leaf, with specific suppression of accumulation of the homologous viral RNA 1, and by the absence of systemic infection. We show that the suppression of viral RNA 1 occurs in protoplasts from resistant transgenic plants and therefore is not due to a host response activated by the cell-to-cell spread of virus. In contrast, suppression of Tobacco rattle virus vectors carrying CMV RNA 1 sequences did not occur in protoplasts from resistant plants. Furthermore, steady-state levels of transgene mRNA 1 were higher in resistant than in susceptible lines. Thus, the data indicate that sequence homology is not sufficient to induce suppression. Grafting experiments using transgenic resistant or susceptible rootstocks and scions demonstrated that the resistance mechanism exhibited an additional barrier to phloem entry, preventing CMV from moving a long distance in resistant plants. On the other hand, virus from susceptible rootstocks could systemically infect grafted resistant scions via the phloem. Analysis of viral RNA accumulation in the infected scions showed that the mechanism that suppresses the accumulation of viral RNA 1 at the single-cell level was overcome. The data indicate that this transgene-mediated systemic resistance probably is not based on a posttranscriptional gene-silencing mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.