ArticleJournal of virology2001
The conserved serine 177 in the delta antigen of hepatitis delta virus is one putative phosphorylation site and is required for efficient viral RNA replication.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Human and pathogen-encoded circular RNAs in viral infections: insights into functions and therapeutic opportunities.Human molecular genetics · 2025Review
- Comparative analysis of human, rodent and snake deltavirus replication.PLoS pathogens · 2024Article
- Article
- Review
- Multiple Regions Drive Hepatitis Delta Virus Proliferation and Are Therapeutic Targets.Frontiers in microbiology · 2022Review
- Characterization of hepatitis B and delta coinfection in Israel.BMC infectious diseases · 2018Article
- Hepatitis delta: virological and clinical aspects.Virology journal · 2017Review
- A novel toolbox for the in vitro assay of hepatitis D virus infection.Scientific reports · 2017Article
- Hepatitis D Virus Replication.Cold Spring Harbor perspectives in medicine · 2015Review
- Hepatitis D virus infection, replication and cross-talk with the hepatitis B virus.World journal of gastroenterology · 2014Article
- Phosphorylation of serine 177 of the small hepatitis delta antigen regulates viral antigenomic RNA replication by interacting with the processive RNA polymerase II.Journal of virology · 2010Article
- Article
- Modification of small hepatitis delta virus antigen by SUMO protein.Journal of virology · 2010Article
- Hepatitis delta virus RNA replication.Viruses · 2009Article
- ERK1/2-mediated phosphorylation of small hepatitis delta antigen at serine 177 enhances hepatitis delta virus antigenomic RNA replication.Journal of virology · 2008Article
- Transcription of subgenomic mRNA of hepatitis delta virus requires a modified hepatitis delta antigen that is distinct from antigenomic RNA synthesis.Journal of virology · 2008Article
- Nucleolar targeting of hepatitis delta antigen abolishes its ability to initiate viral antigenomic RNA replication.Journal of virology · 2008Article
- RNA replication without RNA-dependent RNA polymerase: surprises from hepatitis delta virus.Journal of virology · 2005Review
- Roles of carboxyl-terminal and farnesylated residues in the functions of the large hepatitis delta antigen.Journal of virology · 2005Article
- Genomic but not antigenomic hepatitis delta virus RNA is preferentially exported from the nucleus immediately after synthesis and processing.Journal of virology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatitis delta virus (HDV) small delta antigen (S-HDAg) plays a critical role in virus replication. We previously demonstrated that the S-HDAg phosphorylation occurs on both serine and threonine residues. However, their biological significance and the exact phosphorylation sites of S-HDAg are still unknown. In this study, phosphorylated S-HDAg was detected only in the intracellular compartment, not in viral particles. In addition, the number of phosphorylated isoforms of S-HDAg significantly increased with the extent of viral replication in transfection system. Site-directed mutagenesis showed that alanine replacement of serine 177, which is conserved among all the known HDV strains, resulted in reduced phosphorylation of S-HDAg, while the mutation of the other two conserved serine residues (2 and 123) had little effect. The S177A mutant dramatically decreased its capability in assisting HDV RNA replication, with a preferential and profound impairment of the antigenomic RNA replication. Furthermore, the viral RNA editing, a step relying upon antigenomic RNA replication, was also abolished by this mutation. These results suggested that phosphorylation of S-HDAg, with serine 177 as a presumable site, plays a critical role in viral RNA replication, especially in augmenting the replication of antigenomic RNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.