ArticleThe Journal of physiology2001
Differential regulation of G protein-gated inwardly rectifying K(+) channel kinetics by distinct domains of RGS8.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Signaling Specificity and Kinetics of the Human Metabotropic Glutamate Receptors.Molecular pharmacology · 2024Article
- A biochemical mechanism for time-encoding memory formation within individual synapses of Purkinje cells.PloS one · 2021Article
- Roles for Regulator of G Protein Signaling Proteins in Synaptic Signaling and Plasticity.Molecular pharmacology · 2016Review
- GIRK channel modulation by assembly with allosterically regulated RGS proteins.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Regulation of neuronal GABA(B) receptor functions by subunit composition.Nature reviews. Neuroscience · 2012Review
- Molecular organization and dynamics of the melatonin MT₁ receptor/RGS20/G(i) protein complex reveal asymmetry of receptor dimers for RGS and G(i) coupling.The EMBO journal · 2010Article
- Molecular reconstruction of mGluR5a-mediated endocannabinoid signaling cascade in single rat sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Abundance and stability of complexes containing inactive G protein-coupled receptors and G proteins.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Abscisic acid regulation of guard-cell K+ and anion channels in Gbeta- and RGS-deficient Arabidopsis lines.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- R4 RGS proteins: regulation of G-protein signaling and beyond.Pharmacology & therapeutics · 2007Review
- How regulators of G protein signaling achieve selective regulation.Journal of molecular biology · 2007Review
- Molecular markers of neuronal progenitors in the embryonic cerebellar anlage.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Pleiotropic phenotype of a genomic knock-in of an RGS-insensitive G184S Gnai2 allele.Molecular and cellular biology · 2006Article
- Endogenous RGS proteins enhance acute desensitization of GABA(B) receptor-activated GIRK currents in HEK-293T cells.Pflugers Archiv : European journal of physiology · 2005Article
- RGS9-2 modulates D2 dopamine receptor-mediated Ca2+ channel inhibition in rat striatal cholinergic interneurons.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A retinal-specific regulator of G-protein signaling interacts with Galpha(o) and accelerates an expressed metabotropic glutamate receptor 6 cascade.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Agonist unbinding from receptor dictates the nature of deactivation kinetics of G protein-gated K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Distribution of regulator of G protein signaling 8 (RGS8) protein in the cerebellum.Cerebellum (London, England) · 2003Article
- Gating properties of GIRK channels activated by Galpha(o)- and Galpha(i)-coupled muscarinic m2 receptors in Xenopus oocytes: the role of receptor precoupling in RGS modulation.The Journal of physiology · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
1. The contribution of endogenous regulators of G protein signalling (RGS) proteins to G protein modulated inwardly rectifying K(+) channel (GIRK) activation/deactivation was examined by expressing mutants of Galpha(oA) insensitive to both pertussis toxin (PTX) and RGS proteins in rat sympathetic neurons. 2. GIRK channel modulation was reconstituted in PTX-treated rat sympathetic neurons following heterologous expression of G protein subunits. Under these conditions, noradrenaline-evoked GIRK channel currents displayed: (1) a prominent lag phase preceding activation, (2) retarded activation and deactivation kinetics, and (3) a lack of acute desensitization. 3. Unexpectedly, heterologous expression of RGS8 in neurons expressing PTX-i-RGS-insensitive Galpha(oA) shortened the lag phase and restored rapid activation, but retarded the deactivation phase further. These effects were found to arise from the N-terminus, but not the core domain, of RGS8 thus suggesting actions on channel modulation independently of GTPase acceleration. 4. These findings indicate that different domains of RGS8 make distinct contributions to the temporal regulation of GIRK channels. The RGS8 core domain accelerates termination of the G-protein cycle presumably by increasing Galpha GTPase activity. In contrast, the N-terminal domain of RGS8 appears to promote entry into the G protein cycle, possibly by enhancing coupling of receptors to the G protein heterotrimer. Together, these opposing effects should allow for an increase in temporal fidelity without a dramatic decrease in signal strength.
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