ArticleThe EMBO journal2001
Translocation within the acceptor helix of a major tRNA identity determinant.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A naturally occurring mini-alanyl-tRNA synthetase.Communications biology · 2023Article
- The tRNA identity landscape for aminoacylation and beyond.Nucleic acids research · 2023Review
- Structural basis for shape-selective recognition and aminoacylation of a D-armless human mitochondrial tRNA.Nature communications · 2022Article
- Evolutionary Adjustment of tRNA Identity Rules in Bacillariophyta for Recognition by an Aminoacyl-tRNA Synthetase Adds a Facet to the Origin of Diatoms.Journal of molecular evolution · 2022Article
- Gain of C-Ala enables AlaRS to target the L-shaped tRNAAla.Nucleic acids research · 2022Article
- The Evolutionary Fate of Mitochondrial Aminoacyl-tRNA Synthetases in Amitochondrial Organisms.Journal of molecular evolution · 2021Article
- Article
- Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAThe Journal of biological chemistry · 2020Article
- Naturally Occurring tRNAs With Non-canonical Structures.Frontiers in microbiology · 2020Review
- Nonribosomal Peptide Extension by a Peptide Amino-Acyl tRNA Ligase.Journal of the American Chemical Society · 2019Article
- Contribution of a mitochondrial tyrosyl-tRNA synthetase mutation to the phenotypic expression of the deafness-associated tRNAThe Journal of biological chemistry · 2019Article
- The G3-U70-independent tRNA recognition by human mitochondrial alanyl-tRNA synthetase.Nucleic acids research · 2019Article
- A Hypertension-Associated tRNAAla Mutation Alters tRNA Metabolism and Mitochondrial Function.Molecular and cellular biology · 2016Article
- Towards a comprehensive picture of alloacceptor tRNA remolding in metazoan mitochondrial genomes.Nucleic acids research · 2015Article
- Divergent Alanyl-tRNA Synthetase Genes of Vanderwaltozyma polyspora Descended from a Common Ancestor through Whole-Genome Duplication Followed by Asymmetric Evolution.Molecular and cellular biology · 2015Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- Review
- The evolution of the ribosome and the genetic code.Life (Basel, Switzerland) · 2014Article
- Losing the stem-loop structure from metazoan mitochondrial tRNAs and co-evolution of interacting factors.Frontiers in genetics · 2014Review
- Article
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Authors and funding
3 authors.
Funding
Abstract
The genetic code is defined by the specific aminoacylations of tRNAs by aminoacyl-tRNA synthetases. Although the synthetases are widely conserved through evolution, aminoacylation of a given tRNA is often system specific-a synthetase from one source will not acylate its cognate tRNA from another. This system specificity is due commonly to variations in the sequence of a critical tRNA identity element. In bacteria and the cytoplasm of eukaryotes, an acceptor stem G3:U70 base pair marks a tRNA for aminoacylation with alanine. In contrast, Drosophila melanogaster (Dm) mitochondrial (mt) tRNA(Ala) has a G2:U71 but not a G3:U70 pair. Here we show that this translocated G:U and the adjacent G3:C70 are major determinants for recognition by Dm mt alanyl-tRNA synthetase (AlaRS). Additionally, G:U at the 3:70 position serves as an anti-determinant for Dm mt AlaRS. Consequently, the mitochondrial enzyme cannot charge cytoplasmic tRNA(Ala). All insect mitochondrial AlaRSs appear to have split apart recognition of mitochondrial from cytoplasmic tRNA(Ala) by translocation of G:U. This split may be essential for preventing introduction of ambiguous states into the genetic code.
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