ArticleOncogene2001
Involvement of protein kinase Cdelta in contact-dependent inhibition of growth in human and murine fibroblasts.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Modelling the spatiotemporal dynamics of senescent cells in wound healing, chronic wounds, and fibrosis.PLoS computational biology · 2025Article
- Cell-cell contacts prevent t-BuOOH-triggered ferroptosis and cellular damage in vitro by regulation of intracellular calcium.Archives of toxicology · 2024Article
- Ferroptosis Meets Cell-Cell Contacts.Cells · 2021Review
- Sleeping Beauty and the Microenvironment Enchantment: Microenvironmental Regulation of the Proliferation-Quiescence Decision in Normal Tissues and in Cancer Development.Frontiers in cell and developmental biology · 2018Review
- The Hippo-YAP signaling pathway and contact inhibition of growth.Journal of cell science · 2014Article
- Article
- Protein kinases and phosphatases in the control of cell fate.Enzyme research · 2011Article
- The aryl hydrocarbon receptor (AhR) in the regulation of cell-cell contact and tumor growth.Carcinogenesis · 2010Review
- Neutral sphingomyelinase 2 (nSMase2) is a phosphoprotein regulated by calcineurin (PP2B).The Journal of biological chemistry · 2010Article
- Counter regulatory effects of PKCbetaII and PKCdelta on coronary endothelial permeability.Arteriosclerosis, thrombosis, and vascular biology · 2008Article
- TCDD deregulates contact inhibition in rat liver oval cells via Ah receptor, JunD and cyclin A.Oncogene · 2008Article
- Protein kinase C delta regulates airway mucin secretion via phosphorylation of MARCKS protein.The American journal of pathology · 2007Article
- Protein kinase C family: on the crossroads of cell signaling in skin and tumor epithelium.Journal of cancer research and clinical oncology · 2007Review
- p38alpha MAPK is required for contact inhibition.Oncogene · 2005Article
- Phosphorylation is required for PMA- and cell-cycle-induced degradation of protein kinase Cdelta.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is evidence that protein kinase C delta (PKCdelta) is a tumor suppressor, although its physiological role has not been elucidated so far. Since important anti-proliferative signals are mediated by cell-cell contacts we studied whether PKCdelta is involved in contact-dependent inhibition of growth in human (FH109) and murine (NIH3T3) fibroblasts. Cell-cell contacts were imitated by the addition of glutardialdehyde-fixed cells to sparsely seeded fibroblasts. Downregulation of the PKC isoforms alpha, delta, epsilon, and mu after prolonged treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA, 0.1 microM) resulted in a significant release from contact-inhibition in FH109 cells. Bryostatin 1 selectively prevented TPA-induced PKCdelta-downregulation and reversed TPA-induced release from contact-inhibition arguing for a role of PKCdelta in contact-inhibition. In accordance, the PKCdelta specific inhibitor Rottlerin (1 microM) totally abolished contact-inhibition. Interestingly, immunofluorescence revealed a rapid translocation of PKCdelta to the nucleus when cultures reached confluence with a peak in early-mid G1 phase. Nuclear translocation of PKCdelta in response to cell-cell contacts could also be demonstrated after subcellular fractionation by Western blotting and by measuring PKCdelta-activity after immunoprecipitation. Transient transfection of NIH3T3 cells with a dominant negative mutant of PKCdelta induced a transformed phenotype. We conclude that PKCdelta is involved in contact-dependent inhibition of growth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.