ArticleOncogene2001
Ionizing radiation but not anticancer drugs causes cell cycle arrest and failure to activate the mitochondrial death pathway in MCF-7 breast carcinoma cells.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 81 citations in OpenAlex.
- The dual HDAC/PI3K inhibitor CUDC-907 inhibits the growth and proliferation of MYC-driven Group 3 medulloblastoma.Cell death discovery · 2025Article
- Commentary: Triclabendazole induces pyroptosis by activating caspase-3 to cleave GSDME in breast cancer cells.Frontiers in pharmacology · 2025Article
- Review of Recent Improvements in Carbon Ion Radiation Therapy in the Treatment of Glioblastoma.Advances in radiation oncology · 2024Review
- TCTP regulates genotoxic stress and tumorigenicity via intercellular vesicular signaling.EMBO reports · 2024Article
- Zinc-Substituted Pheophorbide A Is a Safe and Efficient Antivascular Photodynamic Agent.Pharmaceuticals (Basel, Switzerland) · 2022Article
- Metabolic targets of watercress and PEITC in MCF-7 and MCF-10A cells explain differential sensitisation responses to ionising radiation.European journal of nutrition · 2019Article
- Bicalutamide enhances fodrin-mediated apoptosis through calpain in LNCaP.Experimental biology and medicine (Maywood, N.J.) · 2018Article
- Allogeneic hematopoietic cell transplantation as curative therapy for non-transformed follicular lymphomas.Bone marrow transplantation · 2016Article
- Targeting the epigenetics of the DNA damage response in breast cancer.Cell death & disease · 2016Article
- Expression of long non-coding RNA-HOTAIR in oral squamous cell carcinoma Tca8113 cells and its associated biological behavior.American journal of translational research · 2016Article
- Differential expression of long non-coding RNAs during genotoxic stress-induced apoptosis in HeLa and MCF-7 cells.Clinical and experimental medicine · 2013Article
- Enhanced anti-tumour effects of Vinca alkaloids given separately from cytostatic therapies.British journal of pharmacology · 2013Article
- Anti-KIT designer T cells for the treatment of gastrointestinal stromal tumor.Journal of translational medicine · 2013Article
- S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis.Scientific reports · 2013Article
- Induction of p53-inducible microRNA miR-34 by gamma radiation and bleomycin are different.Frontiers in genetics · 2012Article
- Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells.Oncology letters · 2012Article
- Article
- Modeling the role of p53 pulses in DNA damage- induced cell death decision.BMC bioinformatics · 2009Article
- Methyl jasmonate decreases membrane fluidity and induces apoptosis through tumor necrosis factor receptor 1 in breast cancer cells.Anti-cancer drugs · 2008Article
- Role of PP2Calpha in cell growth, in radio- and chemosensitivity, and in tumorigenicity.Molecular cancer · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is considerable evidence that ionizing radiation (IR) and chemotherapeutic drugs mediate apoptosis through the intrinsic death pathway via the release of mitochondrial cytochrome c and activation of caspases -9 and -3. Here we show that MCF-7 cells that lack caspase-3 undergo a caspase-dependent apoptotic cell death in the absence of DNA fragmentation and alpha-fodrin cleavage following treatment with etoposide or doxorubicin, but not after exposure to IR. Re-expression of caspase-3 restored DNA fragmentation and alpha-fodrin cleavage following drug treatment, but it did not alter the radiation-resistant phenotype of these cells. In contrast to the anticancer drugs, IR failed to induce the intrinsic death pathway in MCF-7/casp-3 cells, an event readily observed in IR-induced apoptosis of HeLa cells. Although IR-induced DNA double-strand breaks were repaired with similar efficiencies in all cell lines, cell cycle analyses revealed a persistent G2/M arrest in the two MCF-7 cell lines, but not in HeLa cells. Together, our data demonstrate that caspase-3 is required for DNA fragmentation and alpha-fodrin cleavage in drug-induced apoptosis and that the intrinsic death pathway is fully functional in MCF-7 cells. Furthermore, they show that the radiation-resistant phenotype of MCF-7 cells is not due to the lack of caspase-3, but is caused by the failure of IR to activate the intrinsic death pathway. We propose (1) different signaling pathways are induced by anticancer drugs and IR, and (2) IR-induced G2/M arrest prevents the generation of an apoptotic signal required for the activation of the intrinsic death pathway.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.