ArticleGene therapy2001
Electrotransfer of naked DNA in the skeletal muscles of animal models of muscular dystrophies.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 99 citations in OpenAlex.
- In Vivo Electroporation of Plasmid DNA into the Skeletal Muscle of Dystrophic Mouse Models.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The DUX4-HIF1α Axis in Murine and Human Muscle Cells: A Link More Complex Than Expected.International journal of molecular sciences · 2024Article
- CORP: Gene delivery into murine skeletal muscle using in vivo electroporation.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Review
- Induction of a local muscular dystrophy using electroporation in vivo: an easy tool for screening therapeutics.Scientific reports · 2020Article
- A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle.International journal of molecular sciences · 2019Review
- Current understanding and treatment of cardiac and skeletal muscle pathology in laminin-α2 chain-deficient congenital muscular dystrophy.The application of clinical genetics · 2019Article
- The effects of pprI gene of Deinococcus radiodurans R1 on acute radiation injury of mice exposed to 60Co γ-ray radiation.Oncotarget · 2017Article
- Skeletal muscle regeneration in mice is stimulated by local overexpression of V1a-vasopressin receptor.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Current status of pharmaceutical and genetic therapeutic approaches to treat DMD.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Gene therapy for muscular dystrophies: progress and challenges.Journal of clinical neurology (Seoul, Korea) · 2010Article
- Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle.BMC biotechnology · 2007Article
- Ca(2+) sparks operated by membrane depolarization require isoform 3 ryanodine receptor channels in skeletal muscle.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Therapeutic restoration of dystrophin expression in Duchenne muscular dystrophy.Journal of muscle research and cell motility · 2006Review
- IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors.The Journal of cell biology · 2005Article
- In vivo NMR imaging evaluation of efficiency and toxicity of gene electrotransfer in rat muscle.Gene therapy · 2005Article
- DNA electrotransfer: its principles and an updated review of its therapeutic applications.Gene therapy · 2004Review
- Long-term expression of full-length human dystrophin in transgenic mdx mice expressing internally deleted human dystrophins.Gene therapy · 2004Article
- Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane.The Journal of cell biology · 2003Article
- Progress and prospects: naked DNA gene transfer and therapy.Gene therapy · 2003Review
- High-efficiency plasmid gene transfer into dystrophic muscle.Gene therapy · 2003Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The electrotransfer of naked DNA has recently been adapted to the transduction of skeletal muscle fibers. We investigated the short- and long-term efficacy of this methodology in wild-type animals and in mouse models of congenital muscular dystrophy (dy/dy, dy(2J)/dy(2J)), or Duchenne muscular dystrophy (mdx/mdx). Using a reporter construct, the short-term efficacy of fiber transduction reached 40% and was similar in wild-type, dy/dy and dy(2J)/dy(2J) animals, indicating that ongoing muscle fibrosis was not a major obstacle to the electrotransfer-mediated gene transfer. Although the complete rejection of transduced fibers was observed within 3 weeks in the absence of immunosuppression, the persistency was prolonged over 10 weeks when transient or continuous immunosuppressive regimens were used. Using therapeutic plasmids, we demonstrated that electrotransfer also allowed the transduction of large constructs encoding the laminin alpha2 chain in dy/dy mouse, or a chimeric dystrophin-EGFP protein in mdx/mdx mouse. The correct sarcolemmal localization of these structural proteins demonstrated the functional relevance of their expression in vivo, with a diffusion domain estimated to be 300 to 500 microm. However, degeneration-regeneration events hampered the long-term stability of transduced fibers. Given its efficacy for naked DNA transfer in these models of muscular dystrophies, and despite some limitations, gene electrotransfer methodology should be further explored as a potential avenue for treatment of muscular dystrophies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.