ArticleGene therapy2001
Direct correlation between positron emission tomographic images of two reporter genes delivered by two distinct adenoviral vectors.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 106 citations in OpenAlex.
- Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2019Review
- Modulating the vascular response to limb ischemia: angiogenic and cell therapies.Circulation research · 2015Review
- Somatostatin receptor type 2-based reporter expression after plasmid-based in vivo gene delivery to non-small cell lung cancer.Molecular imaging · 2013Article
- Multimodality molecular imaging of stem cells therapy for stroke.BioMed research international · 2013Review
- Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.Theranostics · 2012Article
- Tracking stem cells for cardiovascular applications in vivo: focus on imaging techniques.Imaging in medicine · 2011Article
- SSTR2-based reporters for assessing gene transfer into non-small cell lung cancer: evaluation using an intrathoracic mouse model.Human gene therapy · 2011Article
- Quantitative PET reporter gene imaging of CD8+ T cells specific for a melanoma-expressed self-antigen.International immunology · 2009Article
- Advances in molecular imaging of pancreatic beta cells.Frontiers in bioscience : a journal and virtual library · 2008Article
- Targeted molecular imaging in oncology: focus on radiation therapy.Seminars in radiation oncology · 2008Review
- Switching on the lights for gene therapy.PloS one · 2007Article
- Gene therapy imaging in patients for oncological applications.European journal of nuclear medicine and molecular imaging · 2005Review
- Human gene therapy and imaging: cardiology.European journal of nuclear medicine and molecular imaging · 2005Review
- Introduction to the background, principles, and state of the art in suicide gene therapy.Molecular biotechnology · 2005Review
- Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography.Cancer gene therapy · 2005Article
- Imaging of human sodium-iodide symporter gene expression mediated by recombinant adenovirus in skeletal muscle of living rats.European journal of nuclear medicine and molecular imaging · 2004Article
- Novel bidirectional vector strategy for amplification of therapeutic and reporter gene expression.Human gene therapy · 2004Article
- Feasibility of sodium/iodide symporter gene as a new imaging reporter gene: comparison with HSV1-tk.European journal of nuclear medicine and molecular imaging · 2004Article
- Gene therapy progress and prospects: noninvasive imaging of gene therapy in living subjects.Gene therapy · 2004Review
- Comparison of [18F]FHBG and [14C]FIAU for imaging of HSV1-tk reporter gene expression: adenoviral infection vs stable transfection.European journal of nuclear medicine and molecular imaging · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Biodistribution, magnitude and duration of a therapeutic transgene's expression may be assessed by linking it to the expression of a positron emission tomography (PET) reporter gene (PRG) and then imaging the PRG's expression by a PET reporter probe (PRP) in living animals. We validate the simple approach of co-administering two distinct but otherwise identical adenoviruses, one expressing a therapeutic transgene and the other expressing the PRG, to track the therapeutic gene's expression. Two PET reporter genes, a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) and dopamine-2 receptor (D(2)R), each regulated by the same cytomegalovirus (CMV) promoter, have been inserted into separate adenoviral vectors (Ad). We demonstrate that cells co-infected with equivalent titers of Ad-CMV-HSV1-sr39tk and Ad-CMV-D(2)R express both reporter genes with good correlation (r(2) = 0.93). Similarly, a high correlation (r(2) = 0.97) was observed between the expression of both PRGs in the livers of mice co-infected via tail-vein injection with equivalent titers of these two adenoviruses. Finally, microPET imaging of HSV1-sr39tk and D(2)R expression with 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl) guanine ([(18)F]FHBG) and 3-(2-[(18)F]fluoroethyl)spiperone ([(18)F]FESP), utilizing several adenovirus-mediated delivery routes, illustrates the feasibility of evaluating relative levels of transgene expression in living animals, using this approach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.