ArticleJournal of clinical microbiology2001
Multiplex PCR protocol for the diagnosis of staphylococcal infection.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 238 citations in OpenAlex.
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- A cross-sectional molecular epidemiological study of biofilm-producing methicillin-resistant Staphylococcus aureus.Medicine · 2025Observational
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- Prevalence, species identification, and antibiotic resistance of Staphylococci in dogs visiting veterinary clinics in Vietnam.PloS one · 2025Article
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- Prevalence of pathogens and antimicrobial resistance of isolated Staphylococcus spp. in bovine mastitis milk in South Korea, 2018-2022.The Journal of veterinary medical science · 2024Article
- Genus-targeted markers for the taxonomic identification and monitoring of coagulase-positive and coagulase-negative Staphylococcus species.World journal of microbiology & biotechnology · 2024Article
- Genomic characteristics of cfr and fexA carrying Staphylococcus aureus isolated from pig carcasses in Korea.Veterinary research · 2024Article
- Antibiogram profiles of pathogenic and commensal bacteria in goat and sheep feces on smallholder farm.Frontiers in antibiotics · 2024Article
- Characterization and pathogenicity of multidrug-resistant coagulase-negative Staphylococci isolates in chickens.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Antimicrobial resistance and virulence of subgingival staphylococci isolated from periodontal health and diseases.Scientific reports · 2023Article
- Resistance profile and biofilm production of Enterococcus spp., Staphylococcus sp., and Streptococcus spp. from dairy farms in southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Antimicrobial Stewardship on Patients with Neutropenia: A Narrative Review Commissioned by Microorganisms.Microorganisms · 2023Review
- Irradiation as a Promising Technology to Improve Bacteriological and Physicochemical Quality of Fish.Microorganisms · 2023Article
- Prevalence and characteristics of foodborne pathogens from slaughtered pig carcasses in Korea.Frontiers in veterinary science · 2023Article
- Development of a Multiplex Polymerase Chain Reaction-Based DNA Lateral Flow Assay as a Point-of-Care Diagnostic for Fast and Simultaneous Detection of MRSA and Vancomycin Resistance in Bacteremia.Diagnostics (Basel, Switzerland) · 2022Article
- Green synthesis of cellulose nanocrystal/ZnO bio-nanocomposites exerting antibacterial activity and downregulating virulence toxigenic genes of food-poisoning bacteria.Scientific reports · 2022Article
- Direct Quantitative Immunochemical Analysis of Autoinducer Peptide IV for Diagnosing and StratifyingACS infectious diseases · 2022Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
We report the development of a multiplex PCR protocol for the diagnosis of staphylococcal infection. The protocol was designed to (i) detect any staphylococcal species to the exclusion of other bacterial pathogens (based on primers corresponding to Staphylococcus-specific regions of the 16S rRNA genes), (ii) distinguish between S. aureus and the coagulase-negative staphylococci (CNS) (based on amplification of the S. aureus-specific clfA gene), and (iii) provide an indication of the likelihood that the staphylococci present in the specimen are resistant to oxacillin (based on amplification of the mecA gene). The expected fragments were amplified from each of 60 staphylococcal isolates (13 oxacillin-resistant S. aureus isolates, 23 oxacillin-sensitive S. aureus isolates, 17 oxacillin-resistant CNS, and 7 oxacillin-sensitive CNS). No amplification products were observed with template DNA from nonstaphylococcal species, and the efficiency of amplification of staphylococcal targets was not adversely affected by the presence of DNA from other bacterial species in the same sample. The utility of the protocol for the analysis of clinical samples was verified by analysis of aliquots taken directly from BacT/Alert blood culture bottles. Of 77 blood cultures tested, only 7 yielded results inconsistent with those of conventional methods of diagnosis and susceptibility testing. Of those, one was identified as a CNS species by PCR and S. aureus by conventional methods. We also identified two isolates that were mecA positive but were oxacillin sensitive according to conventional methods. The other four samples failed to yield any amplification product even with a control set of primers corresponding to a conserved region of the eubacterial rRNA genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.