ArticleJournal of clinical microbiology2001
Identification of novel Helicobacter species in pig stomachs by PCR and partial sequencing.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
- Gastric ulcers in finishing pigs: the evaluation of selected non-dietary risk factors and impact on production performance.Porcine health management · 2024Article
- Presence of potentially novel Helicobacter pylori-like organisms in gastric samples from cats and dogs.Veterinary research · 2023Article
- Perception and Knowledge of Portuguese Veterinarians about the Zoonotic Transmission ofInternational journal of environmental research and public health · 2022Article
- Molecular detection of Helicobacter spp. and Fusobacterium gastrosuis in pigs and wild boars and its association with gastric histopathological alterations.Veterinary research · 2022Article
- The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new specific primers.Scientific reports · 2022Article
- An exploratory study on the presence of Helicobacter heilmannii and Helicobacter billis in the feces of companion dogs.Veterinary medicine and science · 2022Article
- Presence ofJournal of personalized medicine · 2022Article
- Presence ofAnimals : an open access journal from MDPI · 2021Article
- RYGB increases postprandial gastric nesfatin-1 and rapid relieves NAFLD via gastric nerve detachment.PloS one · 2020Article
- Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.Mucosal immunology · 2019Article
- Article
- A 3-year prospective study of the incidence of gastric ulcers in pigs slaughtered at Base Abattoir in Rwanda.Veterinary world · 2017Article
- Helicobacter suis induces changes in gastric inflammation and acid secretion markers in pigs of different ages.Veterinary research · 2017Article
- Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration.Journal of veterinary science · 2010Article
- Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health.Clinical microbiology reviews · 2009Review
- Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.Journal of clinical microbiology · 2008Article
- Experimental infection of pigs with 'Candidatus Helicobacter suis'.Veterinary research communications · 2007Article
- Development of a simple and rapid method based on polymerase chain reaction-based restriction fragment length polymorphism analysis to differentiate Helicobacter, Campylobacter, and Arcobacter species.Current microbiology · 2006Article
- Tetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like gene.Antimicrobial agents and chemotherapy · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evidence of infection with Helicobacter species in pig stomach was investigated by the use of a PCR with Helicobacter genus-specific primers. Forty pig stomachs, each of four different ulcer lesion grades, 0, 1, 2, and 3 in the pars esophagea area, were collected from a slaughterhouse in Minnesota. Of 160 stomach samples examined, 102 (63.8%) were positive by the PCR assay. The 40 samples each of lesion grades 0, 1, 2, and 3 showed 22.5, 52.5, 85.0, and 95.0% PCR-positive results, respectively. There was a significant trend (P < or = 0.01) in the proportions of PCR-positive cases relative to severity of the lesion. About 80% of the 16S rRNA gene was amplified, and PCR-restriction fragment length polymorphism (RFLP) patterns were analyzed. Of 102 PCR-positive samples, the PCR-RFLP patterns resulted in four different types, 32 samples being classified into type MN 1, 16 samples into type MN 2, 43 samples into type MN 3, and 11 samples into type MN 4. When the sequences of each RFLP type were compared to those reported in databases by using BLAST software, types MN 1, MN 2, MN 3, and MN 4 showed homologies of 97.3, 95.4, 96.7, and 99.5% with the 16S ribosomal DNA of Helicobacter flexispira taxon 3, Helicobacter sp. strains MIT 94-022 and MZ 640285, and Helicobacter suis, respectively. None of the 102 samples positive for the Helicobacter genus were positive with a primer set specific for Helicobacter pylori. Attempts to culture the organisms from selected stomachs in vitro were unsuccessful.
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