ArticleJournal of clinical microbiology2001
PCR detection and molecular identification of Chlamydiaceae species.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Swine Conjunctivitis Associated with a Novel Mycoplasma Species Closely Related toPathogens (Basel, Switzerland) · 2020Article
- Isolation of Tetracycline-ResistantAntibiotics (Basel, Switzerland) · 2020Article
- Seroprevalence and molecular characterization of Chlamydia abortus in frozen fetal and placental tissues of aborting ewes in northeastern Algeria.Tropical animal health and production · 2016Article
- Identification and characterization of Chlamydia abortus isolates from yaks in Qinghai, China.BioMed research international · 2015Article
- Molecular identification of chlamydial cause of abortion in small ruminants in Jordan.Tropical animal health and production · 2014Article
- Identification of phylogenetic position in the Chlamydiaceae family for Chlamydia strains released from monkeys and humans with chlamydial pathology.Infectious diseases in obstetrics and gynecology · 2010Article
- Diagnosis of ovine chlamydial abortions by PCR-RFLP performed on vaginal swabs.Veterinary research communications · 2005Article
- Role of Chlamydophila abortus in ovine and caprine abortion in Sardinia, Italy.Veterinary research communications · 2005Article
- Duplex polymerase chain reaction assay to screen for feline herpesvirus-1 and Chlamydophila spp. in mucosal swabs from cats.Veterinary research communications · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent taxonomic developments, based on 16s and 23s rRNA gene sequences, have divided the family Chlamydiaceae into two genera and nine species, of which five have been found to infect humans. Few simple methods are available to detect and identify all species sensitively and specifically. In this study the suitability of the omp2 gene as a target for molecular identification of Chlamydiaceae is demonstrated. Phylogenetic analysis of partial omp2 gene sequences from all nine species agrees with the recently published taxonomic changes based on the ribosomal genes. The use of a family-specific PCR primer pair, which is able to amplify the 5' end of the omp2 gene from all Chlamydiaceae except some Chlamydophila pecorum strains, is described. Identification of all nine species was achieved using restriction fragment length polymorphism analysis with a single enzyme, AluI, confirmed by DNA sequencing. A PCR enzyme-linked oligonucleotide assay was developed which can detect a single chlamydial genome and may be applied to DNA extracts from any specimen or culture for the detection of single or mixed human chlamydial infection.
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Registered trials
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