ArticleJournal of clinical microbiology2001
LightCycler multiplex PCR for the laboratory diagnosis of common viral infections of the central nervous system.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- A Real-Time PCR Assay for Detection of LowFrontiers in microbiology · 2021Article
- Syndrome Evaluation System for Simultaneous Detection Pathogens Causing Acute Encephalitic Syndrome in India, Part-1: Development and Standardization of the Assay.Frontiers in medicine · 2018Article
- Genotypic Identification of Cystoisospora in Immunocompromised Patients Using Tm-Variation Analysis.The Korean journal of parasitology · 2017Article
- Diagnostic Approach to Viral Acute Encephalitis Syndrome (AES) in Paediatric Age Group: A Study from New Delhi.Journal of clinical and diagnostic research : JCDR · 2017Article
- High-throughput multiplexed xMAP Luminex array panel for detection of twenty two medically important mosquito-borne arboviruses based on innovations in synthetic biology.Journal of virological methods · 2015Article
- One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3.BMC veterinary research · 2012Article
- Rapid virological diagnosis of central nervous system infections by use of a multiplex reverse transcription-PCR DNA microarray.Journal of clinical microbiology · 2011Article
- Expression of the SK2 calcium-activated potassium channel is required for cholinergic function in mouse cochlear hair cells.The Journal of physiology · 2008Article
- Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes.BMC bioinformatics · 2007Article
- Real-time PCR in clinical microbiology: applications for routine laboratory testing.Clinical microbiology reviews · 2006Review
- Development of multiplex PCRs for detection of common viral pathogens and agents of congenital infections.Journal of clinical microbiology · 2005Article
- DNA probe array for the simultaneous identification of herpesviruses, enteroviruses, and flaviviruses.Journal of clinical microbiology · 2005Article
- Molecular methods for diagnosis of viral encephalitis.Clinical microbiology reviews · 2004Review
- Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions.Methods in microbiology · 2004Review
- A role for arrays in clinical virology: fact or fiction?Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2004Review
- Detection and differentiation of Mycobacterium tuberculosis and nontuberculous mycobacterial isolates by real-time PCR.Journal of clinical microbiology · 2003Article
- Quantitation of cytomegalovirus (hCMV) DNA and beta-actin DNA by duplex real-time fluorescence PCR in solid organ (liver) transplant recipients.Medical microbiology and immunology · 2003Article
- Aseptic meningitis epidemic during a West Nile virus avian epizootic.Emerging infectious diseases · 2003Article
- Molecular analysis of cerebrospinal fluid in viral diseases of the central nervous system.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2003Review
- Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR.Journal of clinical microbiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A conventional multiplex PCR assay that detects herpes simplex virus type 1 (HSV-1), HSV-2, varicella-zoster virus, and enteroviruses for the diagnosis of central nervous system infections was modified to be performed using the LightCycler system. The sensitivity of detection of each of the viruses using the LightCycler assay was compared to that of the conventional assay using external quality assessment material. The assays had equivalent sensitivities, but the LightCycler assay was more rapid, reduced the risk of contamination, and used an amplicon detection format that demonstrated greater discrimination than a gel electrophoresis method.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.