ArticleApplied and environmental microbiology2001
Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 139 citations in OpenAlex.
- Simultaneous optimization of lignocellulosic sugar catabolism via systematic laboratory evolution under complex selection pressure.Nature communications · 2026Article
- Engineering Saccharomyces cerevisiae for growth on xylose using an oxidative pathway.Applied microbiology and biotechnology · 2025Article
- Review
- Kinase expression enhances phenolic aldehydes conversion and ethanol fermentability of Zymomonas mobilis.Bioprocess and biosystems engineering · 2022Article
- Systematic improvement of isobutanol production from D-xylose in engineered Saccharomyces cerevisiae.AMB Express · 2019Article
- PKA and HOG signaling contribute separable roles to anaerobic xylose fermentation in yeast engineered for biofuel production.PloS one · 2019Article
- Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts.Frontiers in microbiology · 2018Review
- Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective.Microbial cell factories · 2017Review
- Article
- Pydna: a simulation and documentation tool for DNA assembly strategies using python.BMC bioinformatics · 2015Article
- Reconstitution of a secondary cell wall in a secondary cell wall-deficient Arabidopsis mutant.Plant & cell physiology · 2015Article
- Enhanced expression of genes involved in initial xylose metabolism and the oxidative pentose phosphate pathway in the improved xylose-utilizing Saccharomyces cerevisiae through evolutionary engineering.Journal of industrial microbiology & biotechnology · 2014Article
- Development of a D-xylose fermenting and inhibitor tolerant industrial Saccharomyces cerevisiae strain with high performance in lignocellulose hydrolysates using metabolic and evolutionary engineering.Biotechnology for biofuels · 2013Article
- Article
- Phenotypic selection of a wild Saccharomyces cerevisiae strain for simultaneous saccharification and co-fermentation of AFEX™ pretreated corn stover.Biotechnology for biofuels · 2013Article
- An improved method of xylose utilization by recombinant Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2012Article
- D-Xylulose kinase from Saccharomyces cerevisiae: isolation and characterization of the highly unstable enzyme, recombinantly produced in Escherichia coli.Protein expression and purification · 2011Article
- Chemical and Synthetic Genetic Array Analysis Identifies Genes that Suppress Xylose Utilization and Fermentation in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2011Article
- Bulk segregant analysis by high-throughput sequencing reveals a novel xylose utilization gene from Saccharomyces cerevisiae.PLoS genetics · 2010Article
- Efficient bioethanol production by a recombinant flocculent Saccharomyces cerevisiae strain with a genome-integrated NADP+-dependent xylitol dehydrogenase gene.Applied and environmental microbiology · 2009Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase). Xylose reductase and xylitol dehydrogenase convert xylose to its isomer xylulose. The gene XKS1 encodes the xylulose-phosphorylating enzyme xylulokinase. In this study, we determined the effect of XKS1 overexpression on two different S. cerevisiae host strains, H158 and CEN.PK, also expressing XYL1 and XYL2. H158 has been previously used as a host strain for the construction of recombinant xylose-utilizing S. cerevisiae strains. CEN.PK is a new strain specifically developed to serve as a host strain for the development of metabolic engineering strategies. Fermentation was carried out in defined and complex media containing a hexose and pentose sugar mixture or a birch wood lignocellulosic hydrolysate. XKS1 overexpression increased the ethanol yield by a factor of 2 and reduced the xylitol yield by 70 to 100% and the final acetate concentrations by 50 to 100%. However, XKS1 overexpression reduced the total xylose consumption by half for CEN.PK and to as little as one-fifth for H158. Yeast extract and peptone partly restored sugar consumption in hydrolysate medium. CEN.PK consumed more xylose but produced more xylitol than H158 and thus gave lower ethanol yields on consumed xylose. The results demonstrate that strain background and modulation of XKS1 expression are important for generating an efficient xylose-fermenting recombinant strain of S. cerevisiae.
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