ArticleApplied and environmental microbiology2001
Rapid and efficient extraction method for reverse transcription-PCR detection of hepatitis A and Norwalk-like viruses in shellfish.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 113 citations in OpenAlex.
- Randomized, double-blinded clinical trial for human norovirus inactivation in oysters by high hydrostatic pressure processing.Applied and environmental microbiology · 2011Trial
- Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology.Food and environmental virology · 2023Article
- Current and Emerging Technologies for the Detection of Norovirus from Shellfish.Foods (Basel, Switzerland) · 2019Article
- Food-Borne Viruses in Shellfish: Investigation on Norovirus and HAV Presence in Apulia (SE Italy).Food and environmental virology · 2017Article
- Temperature-Dependent Persistence of Human Norovirus Within Oysters (Crassostrea virginica).Food and environmental virology · 2016Article
- Outbreak of Hepatitis A in Italy Associated with Frozen Redcurrants Imported from Poland: A Case Study.Food and environmental virology · 2015Article
- Evaluation of Immunomagnetic Separation Method for the Recovery of Hepatitis A Virus and GI.1 and GII.4 Norovirus Strains Seeded on Oyster and Mussel.Food and environmental virology · 2014Article
- A rapid single-tube protocol for HAV detection by nested real-time PCR.Food and environmental virology · 2014Article
- Hemocytes are sites of enteric virus persistence within oysters.Applied and environmental microbiology · 2011Article
- Detection of hepatitis a virus from oyster by nested PCR using efficient extraction and concentration method.Journal of microbiology (Seoul, Korea) · 2008Article
- Two-log increase in sensitivity for detection of norovirus in complex samples by concentration with porcine gastric mucin conjugated to magnetic beads.Applied and environmental microbiology · 2008Article
- Human noroviruses in swine and cattle.Emerging infectious diseases · 2007Article
- Diagnosis of hepatitis a virus infection: a molecular approach.Clinical microbiology reviews · 2006Review
- High-pressure inactivation of hepatitis A virus within oysters.Applied and environmental microbiology · 2005Article
- Virus-contaminated oysters: a three-month monitoring of oysters imported to Switzerland.Applied and environmental microbiology · 2003Article
- Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation.World journal of gastroenterology · 2002Article
- Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness.Applied and environmental microbiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As part of an effort to develop a broadly applicable test for Norwalk-like viruses and hepatitis A virus (HAV) in shellfish, a rapid extraction method that is suitable for use with one-step reverse transcription (RT)-PCR-based detection methods was developed. The method involves virus extraction using a pH 9.5 glycine buffer, polyethylene glycol (PEG) precipitation, Tri-reagent, and purification of viral poly(A) RNA by using magnetic poly(dT) beads. This glycine-PEG-Tri-reagent-poly(dT) method can be performed in less than 8 h on hard-shell clams (Mercenaria mercenaria) and Eastern oysters (Crassostrea virginica) and, when coupled with RT-PCR-based detection, can yield results within 24 h. Observed sensitivities for seeded shellfish extracts are as low as 0.015 PFU of HAV and 22.4 RT-PCR50 units for Norwalk virus. Detection of HAV in live oysters experimentally exposed to contaminated seawater is also demonstrated. An adaptation of this method was used to identify HAV in imported clams (tentatively identified as Ruditapes philippinarum) implicated in an outbreak of food-borne viral illness. All of the required reagents are commercially available. This method should facilitate the implementation of RT-PCR testing of commercial shellfish.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.