ArticleApplied and environmental microbiology2001
Identification and characterization of a chitinase antigen from Pseudomonas aeruginosa strain 385.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Production, optimization, characterization, and application of chitinase from Bacillus proteolyticus isolated from the rhizosphere of diseased arecanut palm.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens.PLoS pathogens · 2024Article
- Exploring roles of the chitinase ChiC in modulatingMicrobiology spectrum · 2024Article
- Rice false smut virulence protein subverts host chitin perception and signaling at lemma and palea for floral infection.The Plant cell · 2024Article
- Whole Genome Sequencing and Pan-Genomic Analysis of Multidrug-ResistantMicroorganisms · 2023Article
- Maize Endochitinase Expression in Response to Fall Armyworm Herbivory.Journal of chemical ecology · 2021Article
- APathogens (Basel, Switzerland) · 2020Article
- APlant physiology · 2019Article
- Antibiotic discovery throughout the Small World Initiative: A molecular strategy to identify biosynthetic gene clusters involved in antagonistic activity.MicrobiologyOpen · 2017Article
- Environmental Pseudomonads Inhibit Cystic Fibrosis Patient-Derived Pseudomonas aeruginosa.Applied and environmental microbiology · 2017Article
- Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato.Frontiers in plant science · 2015Article
- Refolding of β-stranded class I chitinases of Hippophae rhamnoides enhances the antifreeze activity during cold acclimation.PloS one · 2014Article
- Production of antifungal chitinase by Aspergillus niger LOCK 62 and its potential role in the biological control.Current microbiology · 2012Article
- Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB.Journal of bacteriology · 2010Article
- The chitobiose transporter, chbC, is required for chitin utilization in Borrelia burgdorferi.BMC microbiology · 2010Article
- Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A chitinase antigen has been identified in Pseudomonas aeruginosa strain 385 using sera from animals immunized with a whole-cell vaccine. The majority of the activity was shown to be in the cytoplasm, with some activity in the membrane fraction. The chitinase was not secreted into the culture medium. Purification of the enzyme was achieved by exploiting its binding to crab shell chitin. The purified enzyme had a molecular mass of 58 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a pI of 5.2. NH2-terminal amino acid sequencing revealed two sequences of M(I/L)RID and (Q/M/V)AREDAAAAM that gave an exact match to sequences in a translated putative open reading frame from the P. aeruginosa genome. The chitinase was active against chitin azure, ethylene glycol chitin, and colloidal chitin. It did not display any lysozyme activity. Using synthetic 4-methylumbelliferyl chitin substrates, it was shown to be an endochitinase. The Km and kcat for 4-nitrophenyl-beta-D-N,N'-diacetylchitobiose were 4.28 mM and 1.7 s(-1) respectively, and for 4-nitrophenyl-beta-D-N,N',N"-triacetylchitotriose, they were 0.48 mM and 0.16 s(-1) respectively. The pH optimum was determined to be pH 6.75, and 90% activity was maintained over the pH range 6.5 to 7.1. The enzyme was stable over the pH range 5 to 10 for 3 h and to temperatures up to 50 degrees C for 30 min. The chitinase bound strongly to chitin, chitin azure, colloidal chitin, lichenan, and cellulose but poorly to chitosan, xylan, and heparin. It is suggested that the chitinase functions primarily as a chitobiosidase, removing chitobiose from the nonreducing ends of chitin and chitin oligosaccharides.
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