ArticleApplied and environmental microbiology2001
Construction and environmental release of a Sinorhizobium meliloti strain genetically modified to be more competitive for alfalfa nodulation.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 69 citations in OpenAlex.
- Non-Thermal Plasma-Mediated Redox Signaling and Microbiome Interactions for Abiotic Stress Adaptation: Molecular Insights and Future Prospects for Sustainable Agriculture.International journal of molecular sciences · 2026Review
- Design and regulation of engineered bacteria for environmental release.Nature microbiology · 2025Review
- Engineering D-glucose utilization in Azospirillum brasilense Sp7 promotes rice root colonization.Applied microbiology and biotechnology · 2022Article
- Current Techniques to Study Beneficial Plant-Microbe Interactions.Microorganisms · 2022Review
- Legume-rhizobium dance: an agricultural tool that could be improved?Microbial biotechnology · 2021Review
- Article
- Molecular Biology in the Improvement of Biological Nitrogen Fixation by Rhizobia and Extending the Scope to Cereals.Microorganisms · 2021Review
- Transcriptomic Responses ofFrontiers in microbiology · 2021Article
- A novel biosensor to monitor proline in pea root exudates and nodules under osmotic stress and recovery.Plant and soil · 2020Article
- Enhancement of vitality and activity of a plant growth-promoting bacteria (PGPB) by atmospheric pressure non-thermal plasma.Scientific reports · 2019Article
- Soybean-Nodulating Strains With Low Intrinsic Competitiveness for Nodulation, Good Symbiotic Performance, and Stress-Tolerance Isolated From Soybean-Cropped Soils in Argentina.Frontiers in microbiology · 2019Article
- Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates.Scientific reports · 2017Article
- Proline utilization system is required for infection by the pathogenic α-proteobacterium Brucella abortus.Microbiology (Reading, England) · 2017Article
- Paracoccus denitrificans possesses two BioR homologs having a role in regulation of biotin metabolism.MicrobiologyOpen · 2015Article
- Article
- Engineering rhizobial bioinoculants: a strategy to improve iron nutrition.TheScientificWorldJournal · 2013Review
- Brucella BioR regulator defines a complex regulatory mechanism for bacterial biotin metabolism.Journal of bacteriology · 2013Article
- Profligate biotin synthesis in α-proteobacteria - a developing or degenerating regulatory system?Molecular microbiology · 2013Article
- Comparative study of wild and transformed salt tolerant bacterial strains on Triticum aestivum growth under salt stress.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2010Article
- Long-term field release of bioluminescent Sinorhizobium meliloti strains to assess the influence of a recA mutation on the strains' survival.Microbial ecology · 2006Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Highly efficient nitrogen-fixing strains selected in the laboratory often fail to increase legume production in agricultural soils containing indigenous rhizobial populations because they cannot compete against these populations for nodule formation. We have previously demonstrated, with a Sinorhizobium meliloti PutA- mutant strain, that proline dehydrogenase activity is required for colonization and therefore for the nodulation efficiency and competitiveness of S. meliloti on alfalfa roots (J. I. Jiménez-Zurdo, P. van Dillewijn, M. J. Soto, M. R. de Felipe, J. Olivares, and N. Toro, Mol. Plant-Microbe Interact. 8:492-498, 1995). In this work, we investigated whether the putA gene could be used as a means of increasing the competitiveness of S. meliloti strains. We produced a construct in which a constitutive promoter was placed 190 nucleotides upstream from the start codon of the putA gene. This resulted in an increase in the basal expression of this gene, with this increase being even greater in the presence of the substrate proline. We found that the presence of multicopy plasmids containing this putA gene construct increased the competitiveness of S. meliloti in microcosm experiments in nonsterile soil planted with alfalfa plants subjected to drought stress only during the first month. We investigated whether this construct also increased the competitiveness of S. meliloti strains under agricultural conditions by using it as the inoculum in a contained field experiment at León, Spain. We found that the frequency of nodule occupancy was higher with inoculum containing the modified putA gene for samples that were analyzed after 34 days but not for samples that were analyzed later.
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