ArticleNucleic acids research2001
Linear 2' O-Methyl RNA probes for the visualization of RNA in living cells.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 176 citations in OpenAlex.
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- Biologically stable threose nucleic acid-based probes for real-time microRNA detection and imaging in living cells.Molecular therapy. Nucleic acids · 2022Article
- Kinetics of heterochiral strand displacement from PNA-DNA heteroduplexes.Nucleic acids research · 2021Article
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- 2'-RSC advances · 2020Article
- Non-coding RNAs in cancer: platforms and strategies for investigating the genomic "dark matter".Journal of experimental & clinical cancer research : CR · 2020Review
- Nucleic-Acid Structures as Intracellular Probes for Live Cells.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- l-DNA-Based Catalytic Hairpin Assembly Circuit.Molecules (Basel, Switzerland) · 2020Article
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
- Heterochiral Nucleic Acid Circuits.Emerging topics in life sciences · 2019Article
- Mirror-Image Oligonucleotides: History and Emerging Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2019Review
- Evaluation of fluorescence in situ hybridization techniques to study long non-coding RNA expression in cultured cells.Nucleic acids research · 2018Article
- Nucleic Acid-Based Nanodevices in Biological Imaging.Annual review of biochemistry · 2016Review
- Imaging viral RNA using multiply labeled tetravalent RNA imaging probes in live cells.Methods (San Diego, Calif.) · 2016Review
- RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian Cells.Current protocols in chemical biology · 2016Article
- Can we observe changes in mRNA "state"? Overview of methods to study mRNA interactions with regulatory proteins relevant in cancer related processes.The Analyst · 2016Review
- The use of nanocrystal quantum dot as fluorophore reporters in molecular beacon-based assays.Nano convergence · 2016Review
- ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues.Nucleic acids research · 2015Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
U1snRNA, U3snRNA, 28 S ribosomal RNA, poly(A) RNA and a specific messenger RNA were visualized in living cells with microinjected fluorochrome-labeled 2' O-Methyl oligoribonucleotides (2' OMe RNA). Antisense 2' OMe RNA probes showed fast hybridization kinetics, whereas conventional oligodeoxyribonucleotide (DNA) probes did not. The nuclear distributions of the signals in living cells were similar to those found in fixed cells, indicating specific hybridization. Cytoplasmic ribosomal RNA, poly(A) RNA and mRNA could hardly be visualized, mainly due to a rapid entrapment of the injected probes in the nucleus. The performance of linear probes was compared with that of molecular beacons, which due to their structure should theoretically fluoresce only upon hybridization. No improvements were achieved however with the molecular beacons used in this study, suggesting opening of the beacons by mechanisms other than hybridization. The results show that linear 2' OMe RNA probes are well suited for RNA detection in living cells, and that these probes can be applied for dynamic studies of highly abundant nuclear RNA. Furthermore, it proved feasible to combine RNA detection with that of green fluorescent protein-labeled proteins in living cells. This was applied to show co-localization of RNA with proteins and should enable RNA-protein interaction studies.
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