ArticleNucleic acids research2001
5'-bis-pyrenylated oligonucleotides displaying excimer fluorescence provide sensitive probes of RNA sequence and structure.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 76 citations in OpenAlex.
- Novel pyrene-calix[4]arene derivatives as highly sensitive sensors for nucleotides, DNA and RNA.RSC advances · 2023Article
- Novel Tripodal Polyamine Tris-Pyrene: DNA/RNA Binding and Photodynamic Antiproliferative Activity.Pharmaceutics · 2023Article
- Methyl Viologens of Bis-(4'-Pyridylethynyl)Arenes - Structures, Photophysical and Electrochemical Studies, and their Potential Application in Biology.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Impact of the Histidine-Triazole and Tryptophan-Pyrene Exchange in the WHW Peptide: Cu(II) Binding, DNA/RNA Interactions and Bioactivity.International journal of molecular sciences · 2022Article
- Bis-Pyrene Photo-Switch Open- and Closed-Form Differently Bind to ds-DNA, ds-RNA and Serum Albumin and Reveal Light-Induced Bioactivity.International journal of molecular sciences · 2021Article
- 2- and 2,7-Substituted para-N-Methylpyridinium Pyrenes: Syntheses, Molecular and Electronic Structures, Photophysical, Electrochemical, and Spectroelectrochemical Properties and Binding to Double-Stranded (ds) DNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Recent Advances in Nucleic Acid Targeting Probes and Supramolecular Constructs Based on Pyrene-Modified Oligonucleotides.Molecules (Basel, Switzerland) · 2017Review
- New concepts of fluorescent probes for specific detection of DNA sequences: bis-modified oligonucleotides in excimer and exciplex detection.The Libyan journal of medicine · 2009Article
- Pyrene excimer signaling molecular beacons for probing nucleic acids.Journal of the American Chemical Society · 2008Article
- Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy.Nucleic acids research · 2006Article
- Pyrene is highly emissive when attached to the RNA duplex but not to the DNA duplex: the structural basis of this difference.Nucleic acids research · 2005Article
- Using pyrene-labeled HIV-1 TAR to measure RNA-small molecule binding.Nucleic acids research · 2003Article
- A solid-phase CuAAC strategy for the synthesis of PNA containing nucleobase surrogates.Artificial DNA, PNA & XNAArticle
- Short double-stranded RNA suppresses multiple drug resistance gene expression in tumor cells.Doklady. Biochemistry and biophysicsArticle
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Oligonucleotide conjugates bearing two pyrene residues attached to 5'-phosphate through a phosphoramide bond were synthesised. Fluorescence spectra of the conjugates show a peak typical of monomer emission (lambda(max) 382 nm) and a broad emission peak with lambda(max )476 nm, which indicates the excimer formation between the two pyrene residues. Conjugation of these two pyrene residues to the 5'-phosphate of oligonucleotides does not affect the stabilities of heteroduplexes formed by conjugates with the corresponding linear strands. A monomer fluorescence of the conjugates is considerably affected by the heteroduplex formation allowing the conjugates to be used as fluorescent hybridisation probes. The 5'-bis-pyrenylated oligonucleotides have been successfully used for investigation of affinity and kinetics of antisense oligonucleotides binding to the multidrug resistance gene 1 (PGY1/MDR1) mRNA. The changes of excimer fluorescence of the conjugates occurring during hybridisation depended on the structure of the binding sites: hybridisation to heavily structured parts of RNA resulted in quenching of the excimer fluorescence, while binding to RNA regions with a loose secondary structure was accompanied by an enhancement of the excimer fluorescence. Potentially, these conjugates may be considered as fluorescent probes for RNA structure investigation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.