ArticleNucleic acids research2001
An enhancer element 6 kb upstream of the mouse HNF4alpha1 promoter is activated by glucocorticoids and liver-enriched transcription factors.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 66 citations in OpenAlex.
- Identification of Highly Repetitive Enhancers with Long-range Regulation Potential in Barley via STARR-seq.Genomics, proteomics & bioinformatics · 2024Article
- A potent HNF4α agonist reveals that HNF4α controls genes important in inflammatory bowel disease and Paneth cells.PloS one · 2022Article
- Liver fat storage is controlled by HNF4α through induction of lipophagy and is reversed by a potent HNF4α agonist.Cell death & disease · 2021Article
- TGR5-HNF4α axis contributes to bile acid-induced gastric intestinal metaplasia markers expression.Cell death discovery · 2020Article
- Bmal1 regulates circadian expression of cytochrome P450 3a11 and drug metabolism in mice.Communications biology · 2019Article
- Article
- A Transcriptional Regulatory Network Containing Nuclear Receptors and Long Noncoding RNAs Controls Basal and Drug-Induced Expression of Cytochrome P450s in HepaRG Cells.Molecular pharmacology · 2018Article
- Hnf4α is involved in the regulation of vertebrate LC-PUFA biosynthesis: insights into the regulatory role of Hnf4α on expression of liver fatty acyl desaturases in the marine teleost Siganus canaliculatus.Fish physiology and biochemistry · 2018Article
- Pregnane X Receptor Represses HNF4α Gene to Induce Insulin-Like Growth Factor-Binding Protein IGFBP1 that Alters Morphology of and Migrates HepG2 Cells.Molecular pharmacology · 2015Article
- Identification of the Flavonoid Luteolin as a Repressor of the Transcription Factor Hepatocyte Nuclear Factor 4α.The Journal of biological chemistry · 2015Article
- Cross Talk Between GH-Regulated Transcription Factors HNF6 and CUX2 in Adult Mouse Liver.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Cholesterol-derived glucocorticoids control early fate specification in embryonic stem cells.Stem cell research · 2015Article
- Identification of alverine and benfluorex as HNF4α activators.ACS chemical biology · 2013Article
- The role of microRNAs in hepatocyte nuclear factor-4alpha expression and transactivation.Biochimica et biophysica acta · 2013Article
- HNF4α antagonists discovered by a high-throughput screen for modulators of the human insulin promoter.Chemistry & biology · 2012Article
- ABCC6 as a target in pseudoxanthoma elasticum.Current drug targets · 2011Review
- Maternal diet and aging alter the epigenetic control of a promoter-enhancer interaction at the Hnf4a gene in rat pancreatic islets.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Multiple nuclear receptors may regulate hepatitis B virus biosynthesis during development.The international journal of biochemistry & cell biology · 2011Article
- Transgenic mice with -6A haplotype of the human angiotensinogen gene have increased blood pressure compared with -6G haplotype.The Journal of biological chemistry · 2010Article
- Locus co-occupancy, nucleosome positioning, and H3K4me1 regulate the functionality of FOXA2-, HNF4A-, and PDX1-bound loci in islets and liver.Genome research · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized a 700 bp enhancer element around -6 kb relative to the HNF4alpha1 transcription start. This element increases activity and confers glucocorticoid induction to a heterologous as well as the homologous promoters in differentiated hepatoma cells and is transactivated by HNF4alpha1, HNF4alpha7, HNF1alpha and HNF1beta in dedifferentiated hepatoma cells. A 240 bp sub-region conserves basal and hormone-induced enhancer activity. It contains HNF1, HNF4, HNF3 and C/EBP binding sites as shown by DNase I footprinting and electrophoretic mobility shift assays using nuclear extracts and/or recombinant HNF1alpha and HNF4alpha1. Mutation analyses showed that the HNF1 site is essential for HNF1alpha transactivation and is required for full basal enhancer activity, as is the C/EBP site. Glucocorticoid response element consensus sites which overlap the C/EBP, HNF4 and HNF3 sites are crucial for optimal hormonal induction. We present a model that accounts for weak expression of HNF4alpha1 in the embryonic liver and strong expression in the newborn/adult liver via the binding sites identified in the enhancer.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.