ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Spike frequency decoding and autonomous activation of Ca2+-calmodulin-dependent protein kinase II in dorsal root ganglion neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 98 citations in OpenAlex.
- Exercise increases Ca2+-calmodulin-dependent protein kinase II activity in human skeletal muscle.The Journal of physiology · 2003Trial
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- Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene-Related Peptide.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Activity-Dependent Gene Expression in Neurons.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2021Review
- Structural Insights into the Regulation of CaCold Spring Harbor perspectives in biology · 2020Review
- The neuronal stimulation-transcription coupling map.Current opinion in neurobiology · 2019Review
- Computational Modeling Reveals Frequency Modulation of Calcium-cAMP/PKA Pathway in Dendritic Spines.Biophysical journal · 2019Article
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- Regulated transport of signaling proteins from synapse to nucleus.Current opinion in neurobiology · 2017Review
- Gene networks activated by specific patterns of action potentials in dorsal root ganglia neurons.Scientific reports · 2017Article
- Endothelial mitochondria regulate the intracellular Ca2+ response to fluid shear stress.American journal of physiology. Cell physiology · 2016Article
- Differential CaMKII regulation by voltage-gated calcium channels in the striatum.Molecular and cellular neurosciences · 2015Article
- Differential expression of CaMKII isoforms and overall kinase activity in rat dorsal root ganglia after injury.Neuroscience · 2015Article
- A novel human R25C-phospholamban mutation is associated with super-inhibition of calcium cycling and ventricular arrhythmia.Cardiovascular research · 2015Article
- Splitting hares and tortoises: a classification of neuronal immediate early gene transcription based on poised RNA polymerase II.Neuroscience · 2013Review
- Calmodulin and calmodulin kinase II mediate emergent bursting activity in the brainstem respiratory network (preBötzinger complex).The Journal of physiology · 2013Article
- Structural studies on the regulation of Ca2+/calmodulin dependent protein kinase II.Current opinion in structural biology · 2013Review
- Role of voltage gated Ca2+ channels in rat visceral hypersensitivity change induced by 2,4,6-trinitrobenzene sulfonic acid.Molecular pain · 2013Article
- Persistent inflammation increases GABA-induced depolarization of rat cutaneous dorsal root ganglion neurons in vitro.Neuroscience · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autonomous activation of calcium-calmodulin kinase (CaMKII) has been proposed as a molecular mechanism for decoding Ca(2+) spike frequencies resulting from action potential firing, but this has not been investigated in intact neurons. This was studied in mouse DRG neurons in culture using confocal measurements of [Ca(2+)](i) and biochemical measurements of CaMKII autophosphorylation and autonomous activity. Using electrical stimulation at different frequencies, we find that CaMKII autonomous activity reached near maximal levels after approximately 45 impulses, regardless of firing frequency (1-10 Hz), and autonomous activity declined with prolonged stimulation. Frequency-dependent activation of CaMKII was limited to spike frequencies in the range of 0.1-1 Hz, despite marked increases in [Ca(2+)](i) at higher frequencies (1-30 Hz). The high levels of autonomous activity measured before stimulation and the relatively long duration of Ca(2+) spikes induced by action potentials ( approximately 300 msec) are consistent with the lower frequency range of action potential decoding by CaMKII. The high autonomous activity under basal conditions was associated with extracellular [Ca(2+)], independently from changes in [Ca(2+)](i), and unrelated to synaptic or spontaneous impulse activity. CaMKII autonomous activity in response to brief bursts of action potentials correlated better with the frequency of Ca(2+) transients than with the concentration of [Ca(2+)](i). In conclusion, CaMKII may decode frequency-modulated responses between 0.1 and 1 Hz in these neurons, but other mechanisms may be required to decode higher frequencies. Alternatively, CaMKII may mediate high-frequency responses in subcellular microdomains in which the enzyme is maintained at a low level of autonomous activity or the Ca(2+) transients have faster kinetics.
Indexed as
Identifiers
11517259PMC6763116W1940772596What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.