ArticlePlant molecular biology2001
Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- The Italian Research on the Molecular Characterization of Maize Kernel Development.International journal of molecular sciences · 2022Review
- DNA demethylation affects imprinted gene expression in maize endosperm.Genome biology · 2022Article
- Multi-Locus GWAS of Quality Traits in Bread Wheat: Mining More Candidate Genes and Possible Regulatory Network.Frontiers in plant science · 2020Article
- Article
- Uniparental and transgressive expression of α-zeins in maize endosperm of o2 hybrid lines.PloS one · 2018Article
- Rapid divergence of prolamin gene promoters of maize after gene amplification and dispersal.Genetics · 2012Article
- Chromatin and DNA modifications in the Opaque2-mediated regulation of gene transcription during maize endosperm development.The Plant cell · 2009Article
- Wild-type opaque2 and defective opaque2 polypeptides form complexes in maize endosperm cells and bind the opaque2-zein target site.Plant physiology · 2007Article
- Extensive maternal DNA hypomethylation in the endosperm of Zea mays.The Plant cell · 2004Article
- Arabidopsis MBD proteins show different binding specificities and nuclear localization.Plant molecular biology · 2003Article
- Article
- Computational approaches to identify promoters and cis-regulatory elements in plant genomes.Plant physiology · 2003Review
- Tissue-specific silencing of a transgene in rice.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the maize endosperm, the expression of the subfamily 4 (SF4) of the zein genes is under the transcriptional control of the Opaque2 (O2) basic leucine zipper transcriptional activator, which binds to the O2-box 5'-TCCACGTAGA-3'. Southern experiments showed that the O2-box core sequence ACGT is heavily methylated in sporophytic tissues but becomes hypomethylated in the endosperm. DNA analyses of two inbred lines and their reciprocal crosses indicate that the hypomethylation state is established on the maternal complements of the endosperm. Electrophoretic mobility shift assay (EMSA) and southwestern experiments with endosperm protein extracts and recombinant O2 using oligonucleotides with methylated and unmethylated cytosines in the O2-box indicate an inhibitory effect of modified sequences on O2-binding activity. These results suggest that DNA methylation modulates O2 activity in vivo and shed light on molecular mechanisms involved in the parent-dependent zein gene expression in maize endosperm.
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