ArticleProtein science : a publication of the Protein Society2001
Substitution of conserved methionines by leucines in chloroplast small heat shock protein results in loss of redox-response but retained chaperone-like activity.
Article in Protein science : a publication of the Protein Society, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 29 citations in OpenAlex.
- Redox-regulated methionine oxidation of Arabidopsis thaliana glutathione transferase Phi9 induces H-site flexibility.Protein science : a publication of the Protein Society · 2019Article
- Protein Methionine Sulfoxide Dynamics in Arabidopsis thaliana under Oxidative Stress.Molecular & cellular proteomics : MCP · 2015Article
- The specificity of the interaction between αB-crystallin and desmin filaments and its impact on filament aggregation and cell viability.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- Methionine mutations of outer membrane protein X influence structural stability and beta-barrel unfolding.PloS one · 2013Article
- The small heat shock proteins from Acidithiobacillus ferrooxidans: gene expression, phylogenetic analysis, and structural modeling.BMC microbiology · 2011Article
- Proteome-derived peptide libraries allow detailed analysis of the substrate specificities of N(alpha)-acetyltransferases and point to hNaa10p as the post-translational actin N(alpha)-acetyltransferase.Molecular & cellular proteomics : MCP · 2011Article
- Conformational dependence of 13C shielding and coupling constants for methionine methyl groups.Journal of biomolecular NMR · 2010Article
- Small heat shock proteins prevent aggregation of citrate synthase and bind to the N-terminal region which is absent in thermostable forms of citrate synthase.Extremophiles : life under extreme conditions · 2007Article
- Chemical cross-linking of the chloroplast localized small heat-shock protein, Hsp21, and the model substrate citrate synthase.Protein science : a publication of the Protein Society · 2007Article
- Divergent evolution of the chloroplast small heat shock protein gene in the genera Rhododendron (Ericaceae) and Machilus (Lauraceae).Annals of botany · 2007Article
- Dual role for tomato heat shock protein 21: protecting photosystem II from oxidative stress and promoting color changes during fruit maturation.The Plant cell · 2005Article
- Sulfur assimilation and the role of sulfur in plant metabolism: a survey.Photosynthesis research · 2004Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During evolution of land plants, a specific motif occurred in the N-terminal domain of the chloroplast-localized small heat shock protein, Hsp21: a sequence with highly conserved methionines, which is predicted to form an amphipathic alpha-helix with the methionines situated along one side. The functional role of these conserved methionines is not understood. We have found previously that treatment, which causes methionine sulfoxidation in Hsp21, also leads to structural changes and loss of chaperone-like activity. Here, mutants of Arabidopsis thaliana Hsp21 protein were created by site-directed mutagenesis, whereby conserved methionines were substituted by oxidation-resistant leucines. Mutants lacking the only cysteine in Hsp21 were also created. Protein analyses by nondenaturing electrophoresis, size exclusion chromatography, and circular dichroism proved that sulfoxidation of the four highly conserved methionines (M49, M52, M55, and M59) is responsible for the oxidation-induced conformational changes in the Hsp21 oligomer. In contrast, the chaperone-like activity was not ultimately dependent on the methionines, because it was retained after methionine-to-leucine substitution. The functional role of the conserved methionines in Hsp21 may be to offer a possibility for redox control of chaperone-like activity and oligomeric structure dynamics.
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