ArticleMolecular biology of the cell2001
Conditional mutations in gamma-tubulin reveal its involvement in chromosome segregation and cytokinesis.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 58 citations in OpenAlex.
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- Upregulated TUBG1 expression is correlated with poor prognosis in hepatocellular carcinoma.PeerJ · 2022Article
- Review
- Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles.The Journal of biological chemistry · 2020Article
- TUBG1 missense variants underlying cortical malformations disrupt neuronal locomotion and microtubule dynamics but not neurogenesis.Nature communications · 2019Article
- Review
- Tubulinopathies continued: refining the phenotypic spectrum associated with variants in TUBG1.European journal of human genetics : EJHG · 2018Article
- γ-Tubulin complexes in microtubule nucleation and beyond.Molecular biology of the cell · 2015Article
- Molecular insight into γ-γ tubulin lateral interactions within the γ-tubulin ring complex (γ-TuRC).Journal of computer-aided molecular design · 2014Article
- The dynamics of microtubule minus ends in the human mitotic spindle.Nature cell biology · 2014Article
- NME7 is a functional component of the γ-tubulin ring complex.Molecular biology of the cell · 2014Article
- Mzt1/Tam4, a fission yeast MOZART1 homologue, is an essential component of the γ-tubulin complex and directly interacts with GCP3(Alp6).Molecular biology of the cell · 2013Article
- GTP regulates the microtubule nucleation activity of γ-tubulin.Nature cell biology · 2013Article
- Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.Molecular biology of the cell · 2013Article
- Virtual and biophysical screening targeting the γ-tubulin complex--a new target for the inhibition of microtubule nucleation.PloS one · 2013Article
- The GCP3-interacting proteins GIP1 and GIP2 are required for γ-tubulin complex protein localization, spindle integrity, and chromosomal stability.The Plant cell · 2012Article
- γ-Tubulin 2 nucleates microtubules and is downregulated in mouse early embryogenesis.PloS one · 2012Article
- Feeding cells induced by phytoparasitic nematodes require γ-tubulin ring complex for microtubule reorganization.PLoS pathogens · 2011Article
- The environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin disturbs the establishment and maintenance of cell polarity in preimplantation rat embryos.Biology of reproduction · 2010Article
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
gamma-Tubulin is a conserved essential protein required for assembly and function of the mitotic spindle in humans and yeast. For example, human gamma-tubulin can replace the gamma-tubulin gene in Schizosaccharomyces pombe. To understand the structural/functional domains of gamma-tubulin, we performed a systematic alanine-scanning mutagenesis of human gamma-tubulin (TUBG1) and studied phenotypes of each mutant allele in S. pombe. Our screen, both in the presence and absence of the endogenous S. pombe gamma-tubulin, resulted in 11 lethal mutations and 12 cold-sensitive mutations. Based on structural mapping onto a homology model of human gamma-tubulin generated by free energy minimization, all deleterious mutations are found in residues predicted to be located on the surface, some in positions to interact with alpha- and/or beta-tubulins in the microtubule lattice. As expected, one class of tubg1 mutations has either an abnormal assembly or loss of the mitotic spindle. Surprisingly, a subset of mutants with abnormal spindles does not arrest in M phase but proceeds through anaphase followed by abnormal cytokinesis. These studies reveal that in addition to its previously appreciated role in spindle microtubule nucleation, gamma-tubulin is involved in the coordination of postmetaphase events, anaphase, and cytokinesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.