ArticleMolecular biology of the cell2001
Filopodial initiation and a novel filament-organizing center, the focal ring.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Roles for Ena/VASP proteins in FMNL3-mediated filopodial assembly.Journal of cell science · 2018Article
- Actin filament-microtubule interactions in axon initiation and branching.Brain research bulletin · 2016Review
- In vivo evaluation of an antibacterial coating containing halogenated furanone compound-loaded poly(l-lactic acid) nanoparticles on microarc-oxidized titanium implants.International journal of nanomedicine · 2016Article
- Ena/VASP regulates mDia2-initiated filopodial length, dynamics, and function.Molecular biology of the cell · 2014Article
- Analysis of nuclear actin by overexpression of wild-type and actin mutant proteins.Histochemistry and cell biology · 2014Article
- Physical properties and cellular responses to calcium phosphate coating produced by laser rapid forming on titanium.Lasers in medical science · 2014Article
- Mitochondrial Dynamics in Retinal Ganglion Cell Axon Regeneration and Growth Cone Guidance.Journal of ocular biology · 2013Article
- Mitochondrial dynamics regulate growth cone motility, guidance, and neurite growth rate in perinatal retinal ganglion cells in vitro.Investigative ophthalmology & visual science · 2012Article
- Unraveling the determinants of protrusion formation.International journal of cell biology · 2012Article
- Nanoparticle-mediated signaling endosome localization regulates growth cone motility and neurite growth.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Nerve growth factor induces axonal filopodia through localized microdomains of phosphoinositide 3-kinase activity that drive the formation of cytoskeletal precursors to filopodia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Arp2/3 complex is important for filopodia formation, growth cone motility, and neuritogenesis in neuronal cells.Molecular biology of the cell · 2008Article
- RhoA-kinase and myosin II are required for the maintenance of growth cone polarity and guidance by nerve growth factor.Journal of neurobiology · 2006Article
- The physics of filopodial protrusion.Biophysical journal · 2005Article
- Three functionally distinct adhesions in filopodia: shaft adhesions control lamellar extension.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Regulation of Cdc42-mediated morphological effects: a novel function for p53.The EMBO journal · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
This study examines filopodial initiation and implicates a putative actin filament organizer, the focal ring. Filopodia were optically recorded as they emerged from veils, the active lamellar extensions of growth cones. Motile histories revealed three events that consistently preceded filopodial emergence: an influx of cytoplasm into adjacent filopodia, a focal increase in phase density at veil margins, and protrusion of nubs that transform into filopodia. The cytoplasmic influx probably supplies materials needed for initiation. In correlated time lapse-immunocytochemistry, these focal phase densities corresponded to adhesions. These adhesions persisted at filopodial bases, regardless of subsequent movements. In correlated time lapse-electron microscopy, these adhesion sites contained a focal ring (an oblate, donut-shaped structure approximately 120 nm in diameter) with radiating actin filaments. Filament geometry may explain filopodial emergence at 30 degree angles relative to adjacent filopodia. A model is proposed in which focal rings play a vital role in initiating and stabilizing filopodia: 1) they anchor actin filaments at adhesions, thereby facilitating tension development and filopodial emergence; 2) "axial" filaments connect focal rings to nub tips, thereby organizing filament bundling and ensuring the bundle intersects an adhesion; and 3) "lateral" filaments interconnect focal rings and filament bundles, thereby helping stabilize lamellar margins and filopodia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.