ArticleMolecular biology of the cell2001
Cdc42-dependent modulation of tight junctions and membrane protein traffic in polarized Madin-Darby canine kidney cells.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 128 citations in OpenAlex.
- Tight junction structure, assembly and (dys)function.Nature reviews. Molecular cell biology · 2026Review
- Epithelial polarization in the 3D matrix requires MST3 signaling to regulate ZO-1 position.PloS one · 2023Article
- TRPM8 as an Anti-Tumoral Target in Prostate Cancer Growth and Metastasis Dissemination.International journal of molecular sciences · 2022Article
- Depletion of the apical endosome in response to viruses and bacterial toxins provides cell-autonomous host defense at mucosal surfaces.Cell host & microbe · 2022Article
- CDC42 controlled apical-basal polarity regulates intestinal stem cell to transit amplifying cell fate transition via YAP-EGF-mTOR signaling.Cell reports · 2022Article
- Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals.Molecular biology of the cell · 2019Article
- The scaffolding protein ZO-1 coordinates actomyosin and epithelial apical specializationsThe Journal of biological chemistry · 2018Article
- An emerging role for IQGAP1 in tight junction control.Small GTPases · 2018Review
- Modulating membrane fluidity corrects Batten disease phenotypes in vitro and in vivo.Neurobiology of disease · 2018Article
- Enteropathogenic E. coli effectors EspF and Map independently disrupt tight junctions through distinct mechanisms involving transcriptional and post-transcriptional regulation.Scientific reports · 2018Article
- Cdc42 regulates junctional actin but not cell polarization in theThe Journal of cell biology · 2017Article
- Cdc42 activation couples fluid shear stress to apical endocytosis in proximal tubule cells.Physiological reports · 2017Article
- An Essential Role for Cdc42 in the Functioning of the Adult Mammary Gland.The Journal of biological chemistry · 2016Article
- Potential Use of Autologous Renal Cells from Diseased Kidneys for the Treatment of Renal Failure.PloS one · 2016Article
- IQGAP1 controls tight junction formation through differential regulation of claudin recruitment.Journal of cell science · 2015Article
- The Cdc42 Effector Kinase PAK4 Localizes to Cell-Cell Junctions and Contributes to Establishing Cell Polarity.PloS one · 2015Article
- Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks.PloS one · 2015Article
- MicroRNA-205 targets tight junction-related proteins during urothelial cellular differentiation.Molecular & cellular proteomics : MCP · 2014Article
- Mechanical checkpoint for persistent cell polarization in adhesion-naive fibroblasts.Biophysical journal · 2014Article
- Rab14 regulation of claudin-2 trafficking modulates epithelial permeability and lumen morphogenesis.Molecular biology of the cell · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Polarized epithelial cells maintain the asymmetric composition of their apical and basolateral membrane domains by at least two different processes. These include the regulated trafficking of macromolecules from the biosynthetic and endocytic pathway to the appropriate membrane domain and the ability of the tight junction to prevent free mixing of membrane domain-specific proteins and lipids. Cdc42, a Rho family GTPase, is known to govern cellular polarity and membrane traffic in several cell types. We examined whether this protein regulated tight junction function in Madin-Darby canine kidney cells and pathways that direct proteins to the apical and basolateral surface of these cells. We used Madin-Darby canine kidney cells that expressed dominant-active or dominant-negative mutants of Cdc42 under the control of a tetracycline-repressible system. Here we report that expression of dominant-active Cdc42V12 or dominant-negative Cdc42N17 altered tight junction function. Expression of Cdc42V12 slowed endocytic and biosynthetic traffic, and expression of Cdc42N17 slowed apical endocytosis and basolateral to apical transcytosis but stimulated biosynthetic traffic. These results indicate that Cdc42 may modulate multiple cellular pathways required for the maintenance of epithelial cell polarity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.