ArticleJournal of bacteriology2001
The Brucella suis homologue of the Agrobacterium tumefaciens chromosomal virulence operon chvE is essential for sugar utilization but not for survival in macrophages.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Inability to catabolize galactose leads to increased ability to compete for nodule occupancy in Sinorhizobium meliloti.Journal of bacteriology · 2012Article
- Characterization of the mmsAB-araD1 (gguABC) genes of Agrobacterium tumefaciens.Journal of bacteriology · 2011Article
- Putative quorum-sensing regulator BlxR of Brucella melitensis regulates virulence factors including the type IV secretion system and flagella.Journal of bacteriology · 2008Article
- Release of periplasmic proteins of Brucella suis upon acidic shock involves the outer membrane protein Omp25.Infection and immunity · 2004Article
- Characterization of new members of the group 3 outer membrane protein family of Brucella spp.Infection and immunity · 2003Article
- Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera.Infection and immunity · 2003Article
- Agrobacterium tumefaciens twin-arginine-dependent translocation is important for virulence, flagellation, and chemotaxis but not type IV secretion.Journal of bacteriology · 2003Article
- Comparative proteome analysis of Brucella melitensis vaccine strain Rev 1 and a virulent strain, 16M.Journal of bacteriology · 2002Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella strains possess an operon encoding type IV secretion machinery very similar to that coded by the Agrobacterium tumefaciens virB operon. Here we describe cloning of the Brucella suis homologue of the chvE-gguA-gguB operon of A. tumefaciens and characterize the sugar binding protein ChvE (78% identity), which in A. tumefaciens is involved in virulence gene expression. B. suis chvE is upstream of the putative sugar transporter-encoding genes gguA and gguB, also present in A. tumefaciens, but not adjacent to that of a LysR-type transcription regulator. Although results of Southern hybridization experiments suggested that the gene is present in all Brucella strains, the ChvE protein was detected only in B. suis and Brucella canis with A. tumefaciens ChvE-specific antisera, suggesting that chvE genes are differently expressed in different Brucella species. Analysis of cell growth of B. suis and of its chvE or gguA mutants in different media revealed that ChvE exhibited a sugar specificity similar to that of its A. tumefaciens homologue and that both ChvE and GguA were necessary for utilization of these sugars. Murine or human macrophage infections with B. suis chvE and gguA mutants resulted in multiplication similar to that of the wild-type strain, suggesting that virB expression was unaffected. These data indicate that the ChvE and GguA homologous proteins of B. suis are essential for the utilization of certain sugars but are not necessary for survival and replication inside macrophages.
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