ArticleJournal of bacteriology2001
Mutational analysis of the Rhizobium lupini H13-3 and Sinorhizobium meliloti flagellin genes: importance of flagellin A for flagellar filament structure and transcriptional regulation.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- A novel extracytoplasmic function sigma factor (RpoE6) regulates biogenesis of a minor flagellin and other functions inJournal of bacteriology · 2026Article
- The motility and chemosensory systems of Rhizobium leguminosarum, their role in symbiosis, and link to PTSEnvironmental microbiology · 2024Article
- Motility inMicroorganisms · 2023Article
- Article
- Duplicated Flagellins in Pseudomonas Divergently Contribute to Motility and Plant Immune Elicitation.Microbiology spectrum · 2023Article
- A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum.PloS one · 2023Article
- FliL and its paralog MotF have distinct roles in the stator activity of the Sinorhizobium meliloti flagellar motor.Molecular microbiology · 2022Article
- Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments.Nature communications · 2022Article
- McpT, a Broad-Range Carboxylate Chemoreceptor in Sinorhizobium meliloti.Journal of bacteriology · 2021Article
- Article
- Rhizobial Chemotaxis and Motility Systems at Work in the Soil.Frontiers in plant science · 2021Review
- ExoS/ChvI Two-Component Signal-Transduction System Activated in the Absence of Bacterial Phosphatidylcholine.Frontiers in plant science · 2021Article
- Tyrosine Nitration of Flagellins: a Response of Sinorhizobium meliloti to Nitrosative Stress.Applied and environmental microbiology · 2020Article
- Programmed Proteolysis of Chemotaxis Proteins in Sinorhizobium meliloti: Features in the C-Terminal Region Control McpU Degradation.Journal of bacteriology · 2020Article
- Cellular Stoichiometry of Chemotaxis Proteins inJournal of bacteriology · 2020Article
- The flagella of 'Candidatus Liberibacter asiaticus' and its movement in planta.Molecular plant pathology · 2020Article
- Flagella-Driven Motility of Bacteria.Biomolecules · 2019Review
- Phylogenomic Analyses ofMicroorganisms · 2019Article
- Spatial arrangement of several flagellins within bacterial flagella improves motility in different environments.Nature communications · 2018Article
- Multiple Flagellin Proteins Have Distinct and Synergistic Roles in Agrobacterium tumefaciens Motility.Journal of bacteriology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Complex flagellar filaments are unusual in their fine structure composed of flagellin dimers, in their right-handed helicity, and in their rigidity, which prevents a switch of handedness. The complex filaments of Rhizobium lupini H13-3 and those of Sinorhizobium meliloti are composed of three and four flagellin (Fla) subunits, respectively. The Fla-encoding genes, named flaA through flaD, are separately transcribed from sigma(28)-specific promoters. Mutational analysis of the fla genes revealed that, in both species, FlaA is the principal flagellin and that FlaB, FlaC, and FlaD are secondary. FlaA and at least one secondary Fla protein are required for assembling a functional flagellar filament. Western analysis revealed a ratio close to 1 of FlaA to the secondary Fla proteins (= FlaX) present in wild-type extracts, suggesting that the complex filament is assembled from FlaA-FlaX heterodimers. Whenever a given mutant combination of Fla prevented the assemblage of an intact filament, the biosynthesis of flagellin decreased dramatically. As shown in S. meliloti by reporter gene analysis, it is the transcription of flaA, but not of flaB, flaC, or flaD, that was down-regulated by such abortive combinations of Fla proteins. This autoregulation of flaA is unusual. We propose that any combination of Fla subunits incapable of assembling an intact filament jams the flagellar export channel and thus prevents the escape of an (as yet unidentified) anti-sigma(28) factor that antagonizes the sigma(28)-dependent transcription of flaA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.