ArticleJournal of bacteriology2001
L-threonine export: use of peptides to identify a new translocator from Corynebacterium glutamicum.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 128 citations in OpenAlex.
- Article
- Harnessing prokaryotic amino acid transporters for metabolic engineering: mechanisms and biotechnological applications.Synthetic and systems biotechnology · 2026Review
- Enhanced l-serine synthesis inSynthetic and systems biotechnology · 2025Article
- Impact of exporter proteins and their engineering on the productivity of Corynebacterium.Applied microbiology and biotechnology · 2025Review
- Engineering of L-threonine and L-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening.Bioresources and bioprocessing · 2025Article
- A Novel Lineage of Endosymbiotic Actinomycetales: Genome Reduction and Acquisition of New Functions in Bifidobacteriaceae Associated With Termite Gut Flagellates.Environmental microbiology · 2025Article
- Metabolic engineering of an industrial bacteriumSynthetic and systems biotechnology · 2024Article
- Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic L-threonine producing Corynebacterium glutamicum.Bioresources and bioprocessing · 2024Article
- l-Alanine Exporter AlaE Functions as One of the d-Alanine Exporters inInternational journal of molecular sciences · 2023Article
- Bioprocess Engineering, Transcriptome, and Intermediate Metabolite Analysis of L-Serine High-YieldingMicroorganisms · 2022Article
- Rv3737 is required for Mycobacterium tuberculosis growth in vitro and in vivo and correlates with bacterial load and disease severity in human tuberculosis.BMC infectious diseases · 2022Article
- CRISPR-assisted rational flux-tuning and arrayed CRISPRi screening of an L-proline exporter for L-proline hyperproduction.Nature communications · 2022Article
- High-yield production of L-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- L-Alanine Exporter, AlaE, ofInternational journal of molecular sciences · 2019Article
- A Novel Corynebacterium glutamicum l-Glutamate Exporter.Applied and environmental microbiology · 2018Article
- Metabolite secretion in microorganisms: the theory of metabolic overflow put to the test.Metabolomics : Official journal of the Metabolomic Society · 2018Review
- Systems metabolic engineering strategies for the production of amino acids.Synthetic and systems biotechnology · 2017Review
- Anaerobic growth of Corynebacterium glutamicum via mixed-acid fermentation.Applied and environmental microbiology · 2015Article
- Characterization of the l-alanine exporter AlaE of Escherichia coli and its potential role in protecting cells from a toxic-level accumulation of l-alanine and its derivatives.MicrobiologyOpen · 2015Article
- Effect of biotin on transcription levels of key enzymes and glutamate efflux in glutamate fermentation by Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial mechanisms for the uptake of peptides and their hydrolysis to amino acids are known in great detail, whereas much less is known about the fates of the peptide-derived amino acids. We show that the addition of L-threonine-containing di- or tripeptides results in reduction of the growth of Corynebacterium glutamicum, with concomitant high intracellular accumulation of L-threonine to up to 130 mM. Using transposon mutagenesis and isolation of mutants with increased Thr peptide sensitivity, nine open reading frames (ORFs) were identified, almost all encoding hypothetical proteins of unknown function. Three ORFs encode membrane proteins. Their individual functional characterizations in the wild-type background led to the identification of thrE. Upon thrE overexpression, growth is no longer sensitive to the presence of the Thr peptide, and L-threonine is exported at a rate of 3.8 nmol min(-1) mg of dry weight(-1), whereas the rate of export of a thrE inactivation mutant is reduced to 1.1 nmol min(-1) mg of dry weight(-1). In addition to L-threonine, L-serine is also a substrate for the exporter. The exporter exhibits nine predicted transmembrane-spanning helices with long charged C and N termini and with an amphipathic helix present within the N terminus. All these data suggest that the carrier encoded by thrE serves to export small molecules such as L-threonine and that the carrier is a prototype of a new translocator family. Homologues of ThrE are present in Mycobacterium tuberculosis and Streptomyces coelicolor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.