ArticleJournal of bacteriology2001
Microaerophilic induction of the alpha-crystallin chaperone protein homologue (hspX) mRNA of Mycobacterium tuberculosis.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Proteomics Analysis of Three Different Strains of Mycobacterium tuberculosis under In vitro Hypoxia and Evaluation of Hypoxia Associated Antigen's Specific Memory T Cells in Healthy Household Contacts.Frontiers in microbiology · 2016Article
- Role of Alanine Dehydrogenase of Mycobacterium tuberculosis during Recovery from Hypoxic Nonreplicating Persistence.PloS one · 2016Article
- Polyketide Quinones Are Alternate Intermediate Electron Carriers during Mycobacterial Respiration in Oxygen-Deficient Niches.Molecular cell · 2015Article
- ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase.Journal of bacteriology · 2012Article
- Strain specific transcriptional response in Mycobacterium tuberculosis infected macrophages.Cell communication and signaling : CCS · 2012Article
- Mycobacterium tuberculosis expresses ftsE gene through multiple transcripts.Current microbiology · 2011Article
- The ftsZ Gene of Mycobacterium smegmatis is expressed Through Multiple Transcripts.The open microbiology journal · 2011Article
- Article
- Carbon flux rerouting during Mycobacterium tuberculosis growth arrest.Molecular microbiology · 2010Article
- Different roles of DosS and DosT in the hypoxic adaptation of Mycobacteria.Journal of bacteriology · 2010Article
- Article
- Multiple M. tuberculosis phenotypes in mouse and guinea pig lung tissue revealed by a dual-staining approach.PloS one · 2010Article
- The proteome of Shigella flexneri 2a 2457T grown at 30 and 37 degrees C.Molecular & cellular proteomics : MCP · 2010Article
- Sputum Mycobacterium tuberculosis mRNA as a marker of bacteriologic clearance in response to antituberculosis therapy.Journal of clinical microbiology · 2010Article
- Identification of novel Mycobacterium bovis antigens by dissection of crude protein fractions.Clinical and vaccine immunology : CVI · 2009Article
- Article
- Mycobacterium tuberculosis senses host-derived carbon monoxide during macrophage infection.Cell host & microbe · 2008Article
- Independent loss of immunogenic proteins in Mycobacterium ulcerans suggests immune evasion.Clinical and vaccine immunology : CVI · 2008Article
- The two chorismate mutases from both Mycobacterium tuberculosis and Mycobacterium smegmatis: biochemical analysis and limited regulation of promoter activity by aromatic amino acids.Journal of bacteriology · 2008Article
- Transcriptional characterization of the antioxidant response of Mycobacterium tuberculosis in vivo and during adaptation to hypoxia in vitro.Tuberculosis (Edinburgh, Scotland) · 2008Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Among the products that are expressed when Mycobacterium tuberculosis undergoes hypoxic shiftdown to nonreplicating persistence (NRP) is the alpha-crystallin chaperone protein homologue (Acr). This expression coincides with the previously reported appearance of a respiratory type of nitrate reductase activity, the increase in glycine dehydrogenase activity, and the production of a unique antigen, URB-1. In a timed sampling study, using a slowly stirred oxygen depletion culture model, we have demonstrated that the hspX mRNA that codes for Acr protein as well as the protein itself is induced just as the bacilli enter the microaerophilic NRP stage 1 (NRP-1). In contrast to the induction observed for hspX mRNA, levels of 16S rRNA, fbpB mRNA (encoding the 85B alpha antigen), and aroB mRNA (encoding dehydroquinate synthase) demonstrate relatively small to no change upon entering NRP-1. Acr protein was shown to be identical to URB-1 by Western analysis with anti-URB-1 antibody. The fact that antibody to Acr is found in a high percentage of tuberculosis patients suggests that the hypoxic shiftdown of tubercle bacilli to the NRP state that occurs in vitro, resulting in production of the alpha-crystallin protein, occurs in vivo as well. Simultaneous abrupt increases in hspX mRNA and Acr protein suggest that Acr protein expression is controlled at the level of transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.