ArticleJournal of bacteriology2001
DnaK chaperone-mediated control of activity of a sigma(32) homolog (RpoH) plays a major role in the heat shock response of Agrobacterium tumefaciens.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Expression of Two RpoH Sigma Factors in Sinorhizobium meliloti upon Heat Shock.Microbes and environments · 2017Article
- Purification and biochemical characterization of DnaK and its transcriptional activator RpoH from Neisseria gonorrhoeae.Molecular biology reports · 2014Article
- RpoH(II) activates oxidative-stress defense systems and is controlled by RpoE in the singlet oxygen-dependent response in Rhodobacter sphaeroides.Journal of bacteriology · 2009Article
- sinI- and expR-dependent quorum sensing in Sinorhizobium meliloti.Journal of bacteriology · 2005Article
- The heat shock genes dnaK, dnaJ, and grpE are involved in regulation of putisolvin biosynthesis in Pseudomonas putida PCL1445.Journal of bacteriology · 2005Article
- The RpoH-mediated stress response in Neisseria gonorrhoeae is regulated at the level of activity.Journal of bacteriology · 2004Article
- Replicon-specific regulation of small heat shock genes in Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems.Journal of bacteriology · 2002Article
- HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RpoH (Escherichia coli sigma(32) and its homologs) is the central regulator of the heat shock response in gram-negative proteobacteria. Here we studied salient regulatory features of RpoH in Agrobacterium tumefaciens by examining its synthesis, stability, and activity while increasing the temperature from 25 to 37 degrees C. Heat induction of RpoH synthesis occurred at the level of transcription from an RpoH-dependent promoter, coordinately with that of DnaK, and followed by an increase in the RpoH level. Essentially normal induction of heat shock proteins was observed even with a strain that was unable to increase the RpoH level upon heat shock. Moreover, heat-induced accumulation of dnaK mRNA occurred without protein synthesis, showing that preexisting RpoH was sufficient for induction of the heat shock response. These results suggested that controlling the activity, rather than the amount, of RpoH plays a major role in regulation of the heat shock response. In addition, increasing or decreasing the DnaK-DnaJ chaperones specifically reduced or enhanced the RpoH activity, respectively. On the other hand, the RpoH protein was normally stable and remained stable during the induction phase but was destabilized transiently during the adaptation phase. We propose that the DnaK-mediated control of RpoH activity plays a primary role in the induction of heat shock response in A. tumefaciens, in contrast to what has been found in E. coli.
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