ArticleJournal of bacteriology2001
Gene cluster on pAO1 of Arthrobacter nicotinovorans involved in degradation of the plant alkaloid nicotine: cloning, purification, and characterization of 2,6-dihydroxypyridine 3-hydroxylase.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 80 citations in OpenAlex.
- Horizontal acquisition of nicotine catabolism gene cluster enhances Arthrobacter fitness within tobacco root microbiota.Microbiome · 2026Article
- Genome sequence data of newMicrobiology resource announcements · 2026Article
- Reduction of nicotine content in tobacco through microbial degradation: research progress and potential applications.Biotechnology for biofuels and bioproducts · 2024Review
- Genomic and Metagenomic Insights into the Distribution of Nicotine-degrading Enzymes in Human Microbiota.Current genomics · 2024Article
- A multifunctional flavoprotein monooxygenase HspB for hydroxylation and C-C cleavage of 6-hydroxy-3-succinoyl-pyridine.Applied and environmental microbiology · 2024Article
- Cotinine Hydroxylase CotA Initiates Biodegradation of Wastewater Micropollutant Cotinine inApplied and environmental microbiology · 2021Article
- Structural, Mechanistic, and Functional Insights into anMolecules (Basel, Switzerland) · 2021Article
- Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.Molecules (Basel, Switzerland) · 2021Review
- Paenarthrobacter sp. GOM3 Is a Novel Marine Species With Monoaromatic Degradation Relevance.Frontiers in microbiology · 2021Article
- Volatile organic compounds in the salt-lake sediments of the Tibet Plateau influence prokaryotic diversity and community assembly.Extremophiles : life under extreme conditions · 2020Article
- Biodegradation of persistent environmental pollutants by Arthrobacter sp.Environmental science and pollution research international · 2019Article
- Transcriptome analysis of genes and metabolic pathways associated with nicotine degradation in Aspergillus oryzae 112822.BMC genomics · 2019Article
- Catabolism of 2-Hydroxypyridine by Burkholderia sp. Strain MAK1: a 2-Hydroxypyridine 5-Monooxygenase Encoded byApplied and environmental microbiology · 2018Article
- Insight Into the Diversity and Possible Role of Plasmids in the Adaptation of Psychrotolerant and MetalotolerantFrontiers in microbiology · 2018Article
- The enzymes of microbial nicotine metabolism.Beilstein journal of organic chemistry · 2018Review
- Draft Genome Sequence ofGenome announcements · 2017Article
- Comparative Genomics Reveals Specific Genetic Architectures in Nicotine Metabolism ofFrontiers in microbiology · 2017Article
- Characterization of a Novel Nicotine Degradation Gene ClusterFrontiers in microbiology · 2017Article
- Degradation of Swainsonine by the NADP-Dependent Alcohol Dehydrogenase A1R6C3 in Arthrobacter sp. HW08.Toxins · 2016Article
- A 2-Hydroxypyridine Catabolism Pathway in Rhodococcus rhodochrous Strain PY11.Applied and environmental microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 27,690-bp gene cluster involved in the degradation of the plant alkaloid nicotine was characterized from the plasmid pAO1 of Arthrobacter nicotinovorans. The genes of the heterotrimeric, molybdopterin cofactor (MoCo)-, flavin adenine dinucleotide (FAD)-, and [Fe-S] cluster-dependent 6-hydroxypseudooxynicotine (ketone) dehydrogenase (KDH) were identified within this cluster. The gene of the large MoCo subunit of KDH was located 4,266 bp from the FAD and [Fe-S] cluster subunit genes. Deduced functions of proteins encoded by open reading frames (ORFs) of the cluster were correlated to individual steps in nicotine degradation. The gene for 2,6-dihydroxypyridine 3-hydroxylase was cloned and expressed in Escherichia coli. The purified homodimeric enzyme of 90 kDa contained 2 mol of tightly bound FAD per mol of dimer. Enzyme activity was strictly NADH-dependent and specific for 2,6-dihydroxypyridine. 2,3-Dihydroxypyridine and 2,6-dimethoxypyridine acted as irreversible inhibitors. Additional ORFs were shown to encode hypothetical proteins presumably required for holoenzyme assembly, interaction with the cell membrane, and transcriptional regulation, including a MobA homologue predicted to be specific for the synthesis of the molybdopterin cytidine dinucleotide cofactor.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.