ArticleJournal of bacteriology2001
Feedback regulation of glucose transporter gene transcription in Kluyveromyces lactis by glucose uptake.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- A 2-Deoxyglucose-Resistant Mutant of Saccharomyces cerevisiae Shows Enhanced Maltose Fermentative Ability by the Activation of MAL Genes.Foods (Basel, Switzerland) · 2018Article
- Expansion and Diversification of MFS Transporters inFrontiers in microbiology · 2018Article
- Kinetics and regulation of lactose transport and metabolism in Kluyveromyces lactis JA6.World journal of microbiology & biotechnology · 2014Article
- Proteomic and functional consequences of hexokinase deficiency in glucose-repressible Kluyveromyces lactis.Molecular & cellular proteomics : MCP · 2014Article
- Carbon source dependent promoters in yeasts.Microbial cell factories · 2014Review
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Article
- Expansion of hexose transporter genes was associated with the evolution of aerobic fermentation in yeasts.Molecular biology and evolution · 2011Article
- Crystal structure of hexokinase KlHxk1 of Kluyveromyces lactis: a molecular basis for understanding the control of yeast hexokinase functions via covalent modification and oligomerization.The Journal of biological chemistry · 2010Article
- Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes.Current genetics · 2007Article
- Transcriptomic analysis of extensive changes in metabolic regulation in Kluyveromyces lactis strains.Eukaryotic cell · 2006Article
- Pathways of glucose regulation of monosaccharide transport in grape cells.Plant physiology · 2006Article
- Enolase and glycolytic flux play a role in the regulation of the glucose permease gene RAG1 of Kluyveromyces lactis.Genetics · 2004Article
- Functional characterization of the Frt1 sugar transporter and of fructose uptake in Kluyveromyces lactis.Current genetics · 2003Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the respirofermentative yeast Kluyveromyces lactis, only a single genetic locus encodes glucose transporters that can support fermentative growth. This locus is polymorphic in wild-type isolates carrying either KHT1 and KHT2, two tandemly arranged HXT-like genes, or RAG1, a low-affinity transporter gene that arose by recombination between KHT1 and KHT2. Here we show that KHT1 is a glucose-induced gene encoding a low-affinity transporter very similar to Rag1p. Kht2p has a lower K(m) (3.7 mM) and a more complex regulation. Transcription is high in the absence of glucose, further induced by low glucose concentrations, and repressed at higher glucose concentrations. The response of KHT1 and KHT2 gene regulation to high but not to low concentrations of glucose depends on glucose transport. The function of either Kht1p or Kht2p is sufficient to mediate the characteristic response to high glucose, which is impaired in a kht1 kht2 deletion mutant. Thus, the KHT genes are subject to mutual feedback regulation. Moreover, glucose repression of the endogenous beta-galactosidase (LAC4) promoter and glucose induction of pyruvate decarboxylase were abolished in the kht1 kht2 mutant. These phenotypes could be partially restored by HXT gene family members from Saccharomyces cerevisiae. The results indicate that the specific responses to high but not to low glucose concentrations require a high rate of glucose uptake.
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