ArticleGenetics2001
Bacterial artificial chromosome-based physical map of the rice genome constructed by restriction fingerprint analysis.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Construction and characterization of three wheat bacterial artificial chromosome libraries.International journal of molecular sciences · 2014Article
- Construction and characterization of a bacterial artificial chromosome library for the hexaploid wheat line 92R137.BioMed research international · 2014Article
- Construction of a plant-transformation-competent BIBAC library and genome sequence analysis of polyploid Upland cotton (Gossypium hirsutum L.).BMC genomics · 2013Article
- Construction of BIBAC and BAC libraries from a variety of organisms for advanced genomics research.Nature protocols · 2012 · on this mapArticle
- Preparation of megabase-sized DNA from a variety of organisms using the nuclei method for advanced genomics research.Nature protocols · 2012Article
- Genome physical mapping of polyploids: a BIBAC physical map of cultivated tetraploid cotton, Gossypium hirsutum L.PloS one · 2012Article
- A comparative physical map reveals the pattern of chromosomal evolution between the turkey (Meleagris gallopavo) and chicken (Gallus gallus) genomes.BMC genomics · 2011Article
- BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.).BMC genomics · 2011Article
- A Comparative BAC map for the gilthead sea bream (Sparus aurata L.).Journal of biomedicine & biotechnology · 2011Article
- Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes.Marine biotechnology (New York, N.Y.) · 2010Article
- Numbers of genes in the NBS and RLK families vary by more than four-fold within a plant species and are regulated by multiple factors.Nucleic acids research · 2010Article
- A BAC/BIBAC-based physical map of chickpea, Cicer arietinum L.BMC genomics · 2010Article
- Article
- Construction of a BAC library and a physical map of a major QTL for CBB resistance of common bean (Phaseolus vulgaris L.).Genetica · 2010Article
- Construction and characterization of two bacterial artificial chromosome libraries of grass carp.Marine biotechnology (New York, N.Y.) · 2010Article
- Construction and characterization of a bacterial artificial chromosome (BAC) library of Pacific white shrimp, Litopenaeus vannamei.Marine biotechnology (New York, N.Y.) · 2010Article
- Construction and characterization of a bacterial artificial chromosome library for the A-genome of cotton (G. arboreum L.).Journal of biomedicine & biotechnology · 2010Article
- A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes.BMC genomics · 2009Article
- Recent advances in cotton genomics.International journal of plant genomics · 2008Article
- Construction of BAC and BIBAC libraries from sunflower and identification of linkage group-specific clones by overgo hybridization.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-wide physical mapping with bacteria-based large-insert clones (e.g., BACs, PACs, and PBCs) promises to revolutionize genomics of large, complex genomes. To accelerate rice and other grass species genome research, we developed a genome-wide BAC-based map of the rice genome. The map consists of 298 BAC contigs and covers 419 Mb of the 430-Mb rice genome. Subsequent analysis indicated that the contigs constituting the map are accurate and reliable. Particularly important to proficiency were (1) a high-resolution, high-throughput DNA sequencing gel-based electrophoretic method for BAC fingerprinting, (2) the use of several complementary large-insert BAC libraries, and (3) computer-aided contig assembly. It has been demonstrated that the fingerprinting method is not significantly influenced by repeated sequences, genome size, and genome complexity. Use of several complementary libraries developed with different restriction enzymes minimized the "gaps" in the physical map. In contrast to previous estimates, a clonal coverage of 6.0-8.0 genome equivalents seems to be sufficient for development of a genome-wide physical map of approximately 95% genome coverage. This study indicates that genome-wide BAC-based physical maps can be developed quickly and economically for a variety of plant and animal species by restriction fingerprint analysis via DNA sequencing gel-based electrophoresis.
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