ArticleGenes & development2001
A DNA replication-arrest site RTS1 regulates imprinting by determining the direction of replication at mat1 in S. pombe.
Article in Genes & development, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 121 citations in OpenAlex.
- Can Unicellular Organisms Sequester a Germline? The Yeast-Germline Hypothesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
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- Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo.eLife · 2021Article
- Replication dynamics of recombination-dependent replication forks.Nature communications · 2021Article
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- Asymmetric Histone Inheritance in Asymmetrically Dividing Stem Cells.Trends in genetics : TIG · 2020Review
- Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement.Nature structural & molecular biology · 2019Article
- Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.Nucleic acids research · 2019Article
- Mating-type switching by homology-directed recombinational repair: a matter of choice.Current genetics · 2019Review
- Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.Microbial cell (Graz, Austria) · 2019Review
- Loss of Cohesin Subunit Rec8 Switches Rad51 Mediator Dependence in Resistance to Formaldehyde Toxicity inGenetics · 2018Article
- Repeated evolution of self-compatibility for reproductive assurance.Nature communications · 2018Article
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- Article
- Symmetry from Asymmetry or Asymmetry from Symmetry?Cold Spring Harbor symposia on quantitative biology · 2017Article
- Timeless protection of telomeres.Current genetics · 2016Review
- Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.Genetics · 2015Review
- The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.PloS one · 2015Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mating-type switching in Schizosaccharomyces pombe involves a strand-specific, alkali-labile imprint at the mat1 (mating-type) locus. The imprint is synthesized during replication in a swi1, swi3, and polymerase alpha (swi7) dependent manner and is dependent on mat1 being replicated in a specific direction. Here we show that the direction of replication at mat1 is controlled by a cis-acting polar terminator of replication (RTS1). Two-dimensional gel analysis of replication intermediates reveals that RTS1 only terminates replication forks moving in the centromere-distal direction. A genetic analysis shows that RTS1 optimizes the imprinting process. Transposing the RTS1 element to the distal side of mat1 abolishes imprinting of the native mat1 allele but restores imprinting of an otherwise unimprinted inverted mat1 allele. These data provide conclusive evidence for the "direction of replication model" that explains the asymmetrical switching pattern of S. pombe, and identify a DNA replication-arrest element implicated in a developmental process. Such elements could play a more general role during development and differentiation in higher eukaryotes by regulating the direction of DNA replication at key loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.