ArticleGene therapy2001
Human myeloid dendritic cells transduced with an adenoviral interleukin-10 gene construct inhibit human skin graft rejection in humanized NOD-scid chimeric mice.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 57 citations in OpenAlex.
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- Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: AnExperimental and therapeutic medicine · 2018Article
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- Immunomodulatory Effects of 1,25-Dihydroxyvitamin DJournal of immunology research · 2016Article
- Application of Humanized Mice in Immunological Research.Methods in molecular biology (Clifton, N.J.) · 2016Review
- Cell mediators of autoimmune hepatitis and their therapeutic implications.Digestive diseases and sciences · 2015Review
- DC-Based Immunotherapy Combined with Low-Dose Methotrexate Effective in the Treatment of Advanced CIA in Mice.Journal of immunology research · 2015Article
- The role of dendritic cells in tissue-specific autoimmunity.Journal of immunology research · 2014Review
- Tolerogenic dendritic cell therapy for rheumatoid arthritis: where are we now?Clinical and experimental immunology · 2013Review
- Dendritic cell-based approaches for therapeutic immune regulation in solid-organ transplantation.Journal of transplantation · 2013Review
- Dendritic cell control of tolerogenic responses.Immunological reviews · 2011Review
- Current state of type 1 diabetes immunotherapy: incremental advances, huge leaps, or more of the same?Clinical & developmental immunology · 2011Review
- LPS activation is required for migratory activity and antigen presentation by tolerogenic dendritic cells.Journal of leukocyte biology · 2009Article
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- Immunoregulation of dendritic cells.Clinical medicine & research · 2005Review
- Ovine dendritic cells transduced with an adenoviral CTLA4eEGFP fusion protein construct induce hyporesponsiveness to allostimulation.Immunology · 2004Article
- Interleukin-10 modified dendritic cells induce allo-hyporesponsiveness and prolong small intestine allograft survival.World journal of gastroenterology · 2003Article
- Dendritic cells, T cell tolerance and therapy of adverse immune reactions.Clinical and experimental immunology · 2003Review
- Gene therapy progress and prospects: gene therapy in organ transplantation.Gene therapy · 2003Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human myeloid DC were generated from peripheral blood mononuclear cells by monocyte adhesion and subsequent culture with rhGM-CSF and rhIL-4. We transduced immature (day 5 of culture) myeloid DC with an E1-deleted replication-deficient adenoviral vector encoding the cytokine IL-10 (AdV IL-10) and a control adenovirus MX-17 (AdV MX 17). Human DC transduced with AdV IL-10 showed inhibition of the mixed leukocyte culture, reduced cell surface expression of co-stimulatory molecules (CD80/CD86) and were unable to produce the potent allo-stimulatory cytokine, interleukin-12. In order to test the in vivo properties of these cells a humanized immunodeficient mouse skin transplantation model was developed. Immunodeficient NOD-scid mice were engrafted with human skin, reconstituted via intraperitoneal injection with allogeneic mononuclear cells (MNC) mixed with 1 x 10(6) DC that were autologous to the skin donor and that had been transduced with either AdV IL-10 or AdV MX-17. Skin grafts were removed at day 7 and 14 after reconstitution and studied histologically for evidence of rejection. In animals that received DC modified with AdV IL-10 there was reduced skin graft rejection as characterized by reduced mononuclear cell infiltration and less dermo-epidermal junction destruction compared with those animals that received DC modified with the control virus alone. Injection of equivalent numbers of donor-derived fibroblasts transduced with AdV IL-10 were ineffective at modifying rejection of skin grafts. Immunosuppressive cytokine gene therapy targeting human DC is a novel means of inhibition of the alloimmune response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.