ArticleMolecular and cellular biology2001
Mechanisms controlling subcellular localization of the G(1) cyclins Cln2p and Cln3p in budding yeast.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Modeling the START transition in the budding yeast cell cycle.PLoS computational biology · 2024Article
- Evidence for novel mechanisms that control cell-cycle entry and cell size.Molecular biology of the cell · 2024Article
- Mad3 modulates the GScience advances · 2022Article
- The impact of genomic variation on protein phosphorylation states and regulatory networks.Molecular systems biology · 2022Article
- A hybrid stochastic model of the budding yeast cell cycle.NPJ systems biology and applications · 2020Article
- Proteostasis collapse, a hallmark of aging, hinders the chaperone-Start network and arrests cells in G1.eLife · 2019Article
- A stochastic model of size control in the budding yeast cell cycle.BMC bioinformatics · 2019Article
- Competition in the chaperone-client network subordinates cell-cycle entry to growth and stress.Life science alliance · 2019Article
- Karyopherin Msn5 is involved in a novel mechanism controlling the cellular level of cell cycle regulators Cln2 and Swi5.Cell cycle (Georgetown, Tex.) · 2019Article
- Cdc48/p97 segregase is modulated by cyclin-dependent kinase to determine cyclin fate during G1 progression.The EMBO journal · 2018Article
- Article
- G1 cyclin driven DNA replication.Cell cycle (Georgetown, Tex.) · 2015Article
- Acetyl-CoA induces transcription of the key G1 cyclin CLN3 to promote entry into the cell division cycle in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PP2A(Cdc55) regulates G1 cyclin stability.Cell cycle (Georgetown, Tex.) · 2013Article
- Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets.PloS one · 2013Article
- The CDK Network: Linking Cycles of Cell Division and Gene Expression.Genes & cancer · 2012Article
- Molecular basis of the functional distinction between Cln1 and Cln2 cyclins.Cell cycle (Georgetown, Tex.) · 2012Article
- F-box protein specificity for g1 cyclins is dictated by subcellular localization.PLoS genetics · 2012Article
- A model of yeast cell-cycle regulation based on multisite phosphorylation.Molecular systems biology · 2010Article
- Yeast karyopherin Kap95 is required for cell cycle progression at Start.BMC cell biology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Different G(1) cyclins confer functional specificity to the cyclin-dependent kinase (Cdk) Cdc28p in budding yeast. The Cln3p G(1) cyclin is localized primarily to the nucleus, while Cln2p is localized primarily to the cytoplasm. Both binding to Cdc28p and Cdc28p-dependent phosphorylation in the C-terminal region of Cln2p are independently required for efficient nuclear depletion of Cln2p, suggesting that this process may be physiologically regulated. The accumulation of hypophosphorylated Cln2 in the nucleus is an energy-dependent process, but may not involve the RAN GTPase. Phosphorylation of Cln2p is inefficient in small newborn cells obtained by elutriation, and this lowered phosphorylation correlates with reduced Cln2p nuclear depletion in newborn cells. Thus, Cln2p may have a brief period of nuclear residence early in the cell cycle. In contrast, the nuclear localization pattern of Cln3p is not influenced by Cdk activity. Cln3p localization requires a bipartite nuclear localization signal (NLS) located at the C terminus of the protein. This sequence is required for nuclear localization of Cln3p and is sufficient to confer nuclear localization to green fluorescent protein in a RAN-dependent manner. Mislocalized Cln3p, lacking the NLS, is much less active in genetic assays specific for Cln3p, but more active in assays normally specific for Cln2p, consistent with the idea that Cln3p localization explains a significant part of Clnp functional specificity.
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