ArticleMolecular and cellular biology2001
MGA2 is involved in the low-oxygen response element-dependent hypoxic induction of genes in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 91 citations in OpenAlex.
- Cbf11 and Mga2 function together to activate transcription of lipid metabolism genes and promote mitotic fidelity in fission yeast.PLoS genetics · 2024Article
- Light Stress in Yeasts: Signaling and Responses in Creatures of the Night.International journal of molecular sciences · 2023Review
- Role of spt23 in Saccharomyces cerevisiae thermal tolerance.Applied microbiology and biotechnology · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- Light-Stress Response Mediated by the Transcription FactorFrontiers in microbiology · 2021Article
- Regulation of Ergosterol Biosynthesis inGenes · 2020Review
- Delineating the Rules for Structural Adaptation of Membrane-Associated Proteins to Evolutionary Changes in Membrane Lipidome.Current biology : CB · 2020Article
- Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast.PLoS genetics · 2019Article
- Oxygen-responsive transcriptional regulation of lipid homeostasis in fungi: Implications for anti-fungal drug development.Seminars in cell & developmental biology · 2018Review
- Physiology, ecology and industrial applications of aroma formation in yeast.FEMS microbiology reviews · 2017Review
- Three, two, one yeast fatty acid desaturases: regulation and function.World journal of microbiology & biotechnology · 2017Review
- Engineering Ashbya gossypii strains for de novo lipid production using industrial by-products.Microbial biotechnology · 2017Article
- Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.The Journal of biological chemistry · 2016Article
- Article
- The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.Cell & bioscience · 2012Article
- m:Explorer: multinomial regression models reveal positive and negative regulators of longevity in yeast quiescence.Genome biology · 2012Article
- Kluyveromyces lactis: a suitable yeast model to study cellular defense mechanisms against hypoxia-induced oxidative stress.Oxidative medicine and cellular longevity · 2012Review
- Deletion of a subgroup of ribosome-related genes minimizes hypoxia-induced changes and confers hypoxia tolerance.Physiological genomics · 2011Article
- The Hog1 mitogen-activated protein kinase mediates a hypoxic response in Saccharomyces cerevisiae.Genetics · 2011Article
- Article
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6 authors at 3 institutions in 1 country.
Funding
Abstract
Eukaryotes have the ability to respond to changes in oxygen tension by alterations in gene expression. For example, OLE1 expression in Saccharomyces cerevisiae is upregulated under hypoxic conditions. Previous studies have suggested that the pathway regulating OLE1 expression by unsaturated fatty acids may involve Mga2p and Spt23p, two structurally and functionally related proteins. To define the possible roles of each of these genes on hypoxia-induced OLE1 expression, we examined OLE1 expression under normoxia, hypoxia, and cobalt treatment conditions in Deltamga2 or Deltaspt23 deletion strains. The results of OLE1 promoter-lacZ reporter gene and Northern blot analyses showed that hypoxia- and cobalt-induced OLE1 expression was dramatically decreased in a Deltamga2 strain but not in a Deltaspt23 strain. Further analyses using low-oxygen response element (LORE)-CYC1-lacZ fusion reporter assays and electrophoretic mobility shift assays (EMSAs) demonstrated that MGA2 significantly affects the LORE-dependent hypoxic induction pathway of gene expression. When MGA2 was supplied by a plasmid, the LORE-dependent hypoxia-inducible reporter expression was recovered, as was the hypoxia-inducible complex in EMSAs in the S. cerevisiae Deltamga2 strain. Supershift analysis of EMSAs using crude extracts containing mycMga2p indicated that Mga2p is a component of the LORE-binding complex. Another LORE-dependent, hypoxia-inducible gene, ATF1, was similarly affected in the Deltamga2 strain. These results indicate that MGA2 is required for the LORE-dependent hypoxic gene induction in S. cerevisiae.
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