ArticleBiophysical journal2001
Mechanics and multiple-particle tracking microheterogeneity of alpha-actinin-cross-linked actin filament networks.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 151 citations in OpenAlex.
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- Optical Halo: A Proof of Concept for a New Broadband Microrheology Tool.Micromachines · 2024Article
- Diffusing Wave Microrheology in Polymeric Fluids.Polymers · 2024Article
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Nonlinear Microscale Mechanics of Actin Networks Governed by Coupling of Filament Crosslinking and Stabilization.Polymers · 2022Article
- Tensile force-induced cytoskeletal remodeling: Mechanics before chemistry.PLoS computational biology · 2020Article
- Qualitative analysis of contribution of intracellular skeletal changes to cellular elasticity.Cellular and molecular life sciences : CMLS · 2020Review
- Rheology of the Active Cell Cortex in Mitosis.Biophysical journal · 2016Article
- Imaging of Scleral Collagen Deformation Using Combined Confocal Raman Microspectroscopy and Polarized Light Microscopy Techniques.PloS one · 2016Article
- A microrheological study of hydrogel kinetics and micro-heterogeneity.The European physical journal. E, Soft matter · 2014Article
- Attractive interactions among intermediate filaments determine network mechanics in vitro.PloS one · 2014Article
- The multi-faceted role of the actin cap in cellular mechanosensation and mechanotransduction.Soft matter · 2013Article
- Multiple actin binding domains of Ena/VASP proteins determine actin network stiffening.European biophysics journal : EBJ · 2012Article
- Mapping of bacterial biofilm local mechanics by magnetic microparticle actuation.Biophysical journal · 2012Article
- Morphology and mechanics of chondroid cells from human adipose-derived Stem cells detected by atomic force microscopy.Molecular and cellular biochemistry · 2012Article
- Morphology and viscoelasticity of actin networks formed with the mutually interacting crosslinkers: palladin and alpha-actinin.PloS one · 2012Article
- Nonlinear viscoelasticity of actin transiently cross-linked with mutant α-actinin-4.Journal of molecular biology · 2011Article
- The regulatory action of alpha-actinin on actin filaments is enhanced by cofilin.European biophysics journal : EBJ · 2010Article
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell morphology is controlled by the actin cytoskeleton organization and mechanical properties, which are regulated by the available contents in actin and actin regulatory proteins. Using rheometry and the recently developed multiple-particle tracking method, we compare the mechanical properties and microheterogeneity of actin filament networks containing the F-actin cross-linking protein alpha-actinin. The elasticity of F-actin/alpha-actinin networks increases with actin concentration more rapidly for a fixed molar ratio of actin to alpha-actinin than in the absence of alpha-actinin, for networks of fixed alpha-actinin concentration and of fixed actin concentration, but more slowly than theoretically predicted for a homogeneous cross-linked semiflexible polymer network. These rheological measurements are complemented by multiple-particle tracking of fluorescent microspheres imbedded in the networks. The distribution of the mean squared displacements of these microspheres becomes progressively more asymmetric and wider for increasing concentration in alpha-actinin and, to a lesser extent, for increasing actin concentration, which suggests that F-actin networks become progressively heterogeneous for increasing protein content. This may explain the slower-than-predicted rise in elasticity of F-actin/alpha-actinin networks. Together these in vitro results suggest that actin and alpha-actinin provides the cell with an unsuspected range of regulatory pathways to modulate its cytoskeleton's micromechanics and local organization in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.