ArticleBiophysical journal2001
Temperature and halide dependence of the photocycle of halorhodopsin from Natronobacterium pharaonis.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Molecular mechanism of calcium inhibition in viral channelrhodopsins.Nature communications · 2026Article
- Proteorhodopsin insights into the molecular mechanism of vectorial proton transport.Science advances · 2025Article
- Light-driven anion-pumping rhodopsin with unique cytoplasmic anion-release mechanism.The Journal of biological chemistry · 2024Article
- Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.Advances in experimental medicine and biology · 2021Review
- Molecular mechanism of light-driven sodium pumping.Nature communications · 2020Article
- High-resolution structural insights into the heliorhodopsin family.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Unique structure and function of viral rhodopsins.Nature communications · 2019Article
- Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high ClScientific reports · 2018Article
- Light-Driven Chloride Transport Kinetics of Halorhodopsin.Biophysical journal · 2018Article
- Existence of two O-like intermediates in the photocycle ofBiophysics and physicobiology · 2017Article
- A Unique Light-Driven Proton Transportation Signal in Halorhodopsin from Natronomonas pharaonis.Biophysical journal · 2016Article
- Biophysical Properties of Optogenetic Tools and Their Application for Vision Restoration Approaches.Frontiers in systems neuroscience · 2016Review
- Crystal structures of the L1, L2, N, and O states of pharaonis halorhodopsin.Biophysical journal · 2015Article
- Phototransformation of the red light sensor cyanobacterial phytochrome 2 from Synechocystis species depends on its tongue motifs.The Journal of biological chemistry · 2014Article
- Biodesalination: a case study for applications of photosynthetic bacteria in water treatment.Plant physiology · 2014Review
- Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.Chemical reviews · 2014Review
- Kinetic and thermodynamic analysis of the light-induced processes in plant and cyanobacterial phytochromes.Biophysical journal · 2013Article
- Ser(262) determines the chloride-dependent colour tuning of a new halorhodopsin from Haloquadratum walsbyi.Bioscience reports · 2012Article
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2 authors.
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Abstract
The photocycle kinetics of halorhodopsin from Natronobacterium pharaonis (pHR(575)) was analyzed at different temperatures and chloride concentrations as well as various halides. Over the whole range of modified parameters the kinetics can be adequately modeled with six apparent rate constants. Assuming a model in which the observed rates are assigned to irreversible transitions of a single relaxation chain, six kinetically distinguishable states (P(1-6)) are discernible that are formed from four chromophore states (spectral archetypes S(j): K(570), L(N)(520), O(600), pHR'(575)). Whereas P(1) coincides with K(570) (S(1)), both P(2) and P(3) have identical spectra resembling L(520) (S(2)), thus representing a true spectral silent transition between them. P(4) constitutes a fast temperature-dependent equilibrium between the chromophore states S(2) and S(3) (L(520) and O(600), respectively). The subsequent equilibrium (P(5)) of the same spectral archetypes is only moderately temperature dependent but shows sensitivity toward the type of anion and the chloride concentration. Therefore, S(2) and S(3) occurring in P(4) as well as in P(5) have to be distinguished and are assigned to L(520)<--> O(1)(600) and O(2)(600)<--> N(520) equilibrium, respectively. It is proposed that P(4) and P(5) represent the anion release and uptake steps. Based on the experimental data affinities of the halide binding sites are estimated.
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