ArticleBiophysical journal2001
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 41 citations in OpenAlex.
- Alternative N-terminal regions ofThe Journal of biological chemistry · 2020Article
- Coumarin-Tagged Dinuclear Trithiolato-Bridged Ruthenium(II)⋅Arene Complexes: Photophysical Properties and Antiparasitic Activity.Chembiochem : a European journal of chemical biology · 2020Article
- Convergent Synthesis of Two Fluorescent Ebselen-Coumarin Heterodimers.Pharmaceuticals (Basel, Switzerland) · 2016Article
- The Relay/Converter Interface Influences Hydrolysis of ATP by Skeletal Muscle Myosin II.The Journal of biological chemistry · 2016Article
- Rad51 Nucleoprotein Filament Disassembly Captured Using Fluorescent Plasmodium falciparum SSB as a Reporter for Single-Stranded DNA.PloS one · 2016Article
- Transient signal generation in a self-assembled nanosystem fueled by ATP.Nature communications · 2015Article
- Two Drosophila myosin transducer mutants with distinct cardiomyopathies have divergent ADP and actin affinities.The Journal of biological chemistry · 2011Article
- FRET characterisation for cross-bridge dynamics in single-skinned rigor muscle fibres.European biophysics journal : EBJ · 2011Article
- Fluorescence lifetime imaging reveals that the environment of the ATP binding site of myosin in muscle senses force.Biophysical journal · 2010Article
- Alternative exon 9-encoded relay domains affect more than one communication pathway in the Drosophila myosin head.Journal of molecular biology · 2009Article
- Fluorescent single-stranded DNA binding protein as a probe for sensitive, real-time assays of helicase activity.Biophysical journal · 2008Article
- Direct observation of the mechanochemical coupling in myosin Va during processive movement.Nature · 2008Article
- Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres.Biophysical journal · 2007Article
- A variable domain near the ATP-binding site in Drosophila muscle myosin is part of the communication pathway between the nucleotide and actin-binding sites.Journal of molecular biology · 2007Article
- Smooth muscle myosin: regulation and properties.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2004Review
- Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin.Biophysical journal · 2004Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several coumarin-labeled nucleotides have been synthesized, based on 2'(3')-O-(2-aminoethyl)carbamoyl-ATP (edaATP). The fluorescent coumarins coupled with the free amino group are 7-diethylaminocoumarin-3-carboxylic acid (to give deac-edaATP), coumarin 343 (but-edaATP) and 7-ethylamino-8-bromocoumarin-3-carboxylic acid (mbc-edaATP). The carbamoyl linkage of these nucleotide analogs undergoes interconversion between 2'- and 3'-hydroxyl attachment very slowly, so that the 2'- and 3'-isomers were separated and stored with minimal equilibration. 3'-Deac-edaADP had fluorescence excitation and emission maxima at 430 nm and 477 nm, with a fluorescence quantum yield of 0.012. The equivalent data for 3'-but-edaADP are 445 nm, 494 nm, and 0.51, respectively, and for 3'-mbc-edaADP, 405 nm, 464 nm, and 0.62. The interaction with skeletal myosin subfragment 1 was measured in the absence and presence of actin. In each case the fluorescence was decreased when bound to subfragment 1, 3-fold for 3'-deac-edaADP, 7-fold for 3'-but-edaADP, and 11-fold for 3'-mbc-edaADP. Steady-state ATPase measurements and the kinetics of binding and release of nucleotides were similar to those reported for the natural nucleotide. Large fluorescence changes could be observed for the release of these analogs from actomyosin subfragment 1, enabling a direct measurement of the kinetics of this process. In the case of 3'-deac-edaADP a rate constant of 474 s(-1) was measured (at pH 7.0, 20 degrees C, and low ionic strength).
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