Evidence map›Paper›PMID 11508339›Full record

ArticleThe Histochemical journal2001

The expression of gastric H+-K+-ATPase mRNA and protein in developing rat fundic gland.

D H Yang, S Tsuyama, F Murata

Abstract read
PubMed Publisher
In one paragraph

Article in The Histochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

D H YangDepartment of Anatomy, Kagoshima University Faculty of Medicine, Japan.
S Tsuyama
F Murata
Kagoshima University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The proton pump H+-K+-ATPase is the final common pathway mediating the production and secretion of hydrochloric acid by gastric parietal cells. The present studies were undertaken to examine whether the expression of gastric H+-K+-ATPase mRNA and protein changes are associated with the development of H+-K+-ATPase activity in the rat fundic gland. H+-K+-ATPase activity was examined in rat fundic gland at different stages from gestational day 18.5 to postnatal 8 weeks. The expression of H+-K+-ATPase mRNA was detected by in situ hybridization using a digoxigenin-labelled RNA probe with a tyramide signal amplification system. The expression of H+-K+-ATPase protein was evaluated by immunoblotting and immunohistochemistry using antibodies against H+-K+-ATPase alpha- and beta-subunits. We found that H+-K+-ATPase enzyme activity was detectable from the onset of gland formation (day 19.5 of gestation) and increased with age in the developing rat fundic gland. Expression of mRNA and protein was also discernible at the same time, and a progressive increase in expressions was observed as rats developed. Our results suggested that in developing rat fundic gland, the expression of both mRNA and protein of H+-K+-ATPase increased with age in a manner that parallels the development of H+-K+-ATPase enzyme activity.

Indexed as

4-NitrophenylphosphataseAnimalsBlotting, WesternExocrine GlandsFemaleGastric FundusGastric MucosaGene Expression Regulation, EnzymologicH(+)-K(+)-Exchanging ATPaseImmunohistochemistryIn Situ HybridizationPregnancyProtein BiosynthesisRatsRats, WistarReverse Transcriptase Polymerase Chain Reaction4-NitrophenylphosphataseH(+)-K(+)-Exchanging ATPaseRNA, Messenger

Identifiers

PMID11508339
OpenAlexW32879814

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.