ArticleJournal of virology2001
Biochemical characterization of the helper component of Cauliflower mosaic virus.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- The cauliflower mosaic virus transmission helper protein P2 modifies directly the probing behavior of the aphid vector Myzus persicae to facilitate transmission.PLoS pathogens · 2023Article
- Molecular dynamics investigations for the prediction of molecular interaction of cauliflower mosaic virus transmission helper component protein complex withVirusdisease · 2019Article
- Identification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors.Journal of virology · 2018Article
- Proteomic composition of the acrostyle: Novel approaches to identify cuticular proteins involved in virus-insect interactions.Insect science · 2017Article
- Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern.PloS one · 2015Article
- Structural insights into the molecular mechanisms of cauliflower mosaic virus transmission by its insect vector.Journal of virology · 2010Article
- Plant virus transmission from the insect point of view.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A protein key to plant virus transmission at the tip of the insect vector stylet.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A single amino acid position in the helper component of cauliflower mosaic virus can change the spectrum of transmitting vector species.Journal of virology · 2005Article
- A coiled-coil interaction mediates cauliflower mosaic virus cell-to-cell movement.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The helper component of Cauliflower mosaic virus is encoded by viral gene II. This protein (P2) is dispensable for virus replication but required for aphid transmission. The purification of P2 has never been reported, and hence its biochemical properties are largely unknown. We produced the P2 protein via a recombinant baculovirus with a His tag fused at the N terminus. The fusion protein was purified by affinity chromatography in a soluble and biologically active form. Matrix-assisted laser desorption time-of-flight mass spectrometry demonstrated that P2 is not posttranslationally modified. UV circular dichroism revealed the secondary structure of P2 to be 23% alpha-helical. Most alpha-helices are suggested to be located in the C-terminal domain. Using size exclusion chromatography and aphid transmission testing, we established that the active form of P2 assembles as a huge soluble oligomer containing 200 to 300 subunits. We further showed that P2 can also polymerize as long paracrystalline filaments. We mapped P2 domains involved in P2 self-interaction, presumably through coiled-coil structures, one of which is proposed to form a parallel trimer. These regions have previously been reported to also interact with viral P3, another protein involved in aphid transmission. Possible interference between the two types of interaction is discussed with regard to the biological activity of P2.
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