ArticleBritish journal of cancer2001
Prostate epithelial cell lines form spheroids with evidence of glandular differentiation in three-dimensional Matrigel cultures.
Article in British journal of cancer, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 104 citations in OpenAlex.
- Advances in 3D culture systems for maintaining prostate tissue architecture and functional ex vivo prostate organ culture for biomedical applications.Molecular biology reports · 2026Review
- An orally bioavailable pan-αv/α5β1 integrin antagonist prevents aggressive prostate cancer progression via suppressing both oncogenic signals and CD47-mediated immune escape.Molecular cancer · 2026Article
- Formation of the Vasculogenic Mimicry Phenotype in Melanoma Mel Z Cells Is Coupled with Changes in Inter-Chromosomal Contacts of Developmental Genes with rDNA Clusters.International journal of molecular sciences · 2025Article
- Automated Assessment of Cancer Drug Efficacy On Breast Tumor Spheroids in Aggrewell™400 Plates Using Image Cytometry.Journal of fluorescence · 2022Article
- Updated perspectives on vascular cell specification and pluripotent stem cell-derived vascular organoids for studying vasculopathies.Cardiovascular research · 2022Review
- Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip.Biosensors · 2021Article
- Article
- Three-Dimensional Cell Cultures as an In Vitro Tool for Prostate Cancer Modeling and Drug Discovery.International journal of molecular sciences · 2020Review
- Study on Development of Composite Hydrogels With Tunable Structures and Properties for Tumor-on-a-Chip Research.Frontiers in bioengineering and biotechnology · 2020Article
- DNA-GEL, Novel Nanomaterial for Biomedical Applications and Delivery of Bioactive Molecules.Frontiers in pharmacology · 2020Review
- Hydrogel microenvironments for cancer spheroid growth and drug screening.Science advances · 2018Review
- CK2 abrogates the inhibitory effects of PRH/HHEX on prostate cancer cell migration and invasion and acts through PRH to control cell proliferation.Oncogenesis · 2017Article
- High-Content Monitoring of Drug Effects in a 3D Spheroid Model.Frontiers in oncology · 2017Article
- Article
- Bio-printing cell-laden Matrigel-agarose constructs.Journal of biomaterials applications · 2016Article
- Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro.The Journal of reproduction and development · 2016Article
- Review
- Arrestin2 modulates androgen receptor activation.Oncogene · 2015Article
- Utilizing custom-designed galvanotaxis chambers to study directional migration of prostate cells.Journal of visualized experiments : JoVE · 2014Article
- PRH/HHex inhibits the migration of breast and prostate epithelial cells through direct transcriptional regulation of Endoglin.Oncogene · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Normal (PNT2-C2) and metastatic (PC-3) prostate cell lines were grown in Matrigel to observe the effects on morphology and phenotype in comparison to monolayer culture. In monolayer cultures, PNT2-C2 showed typical round/cuboidal epithelial morphology, with tight cell associations, whereas in Matrigel they formed smooth spheroids, tightly packed with cells. In both monolayer and Matrigel, PNT2-C2 had a differentiated luminal epithelial phenotype with high expression of cytokeratin 8, prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), E-cadherin and desmoglein. In contrast, PC-3 cells possessed an epithelial/mesenchyme morphology in monolayer with loose cell to cell contact and pseudopodial extensions. Immunohistochemical phenotyping indicated the cells were undifferentiated, expressing high levels of vimentin, beta1 integrin, CD44 and low expression of cytokeratin 8. In Matrigel they formed smooth and irregular spheroids, which had a lumen surrounded by a single cell layer. Matrigel also influenced the expression of PSA, PSMA and CD44. These results indicate that Matrigel culture can induce morphological differentiation of prostate cancer cells which initially had a basal phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.