ArticleJournal of the American Society for Mass Spectrometry2001
Elongated conformers of charge states +11 to +15 of bovine ubiquitin studied using ESI-FAIMS-MS.
Article in Journal of the American Society for Mass Spectrometry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 95 citations in OpenAlex.
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- High-Field Asymmetric Waveform Ion Mobility Spectrometry and Native Mass Spectrometry: Analysis of Intact Protein Assemblies and Protein Complexes.Analytical chemistry · 2020Article
- Preferential Ion Microsolvation in Mixed-Modifier Environments Observed Using Differential Mobility Spectrometry.Journal of the American Society for Mass Spectrometry · 2019Article
- Photoelectron Transfer Dissociation Reveals Surprising Favorability of Zwitterionic States in Large Gaseous Peptides and Proteins.Journal of the American Chemical Society · 2017Article
- Reactive Landing of Gramicidin S and Ubiquitin Ions onto Activated Self-Assembled Monolayer Surfaces.Journal of the American Society for Mass Spectrometry · 2017Article
- Differential Mobility Spectrometry-Hydrogen Deuterium Exchange (DMS-HDX) as a Probe of Protein Conformation in Solution.Journal of the American Society for Mass Spectrometry · 2016Article
- To What Extent is FAIMS Beneficial in the Analysis of Proteins?Journal of the American Society for Mass Spectrometry · 2016Article
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- On an aerodynamic mechanism to enhance ion transmission and sensitivity of FAIMS for nano-electrospray ionization-mass spectrometry.Journal of the American Society for Mass Spectrometry · 2014Article
- Experimental evaluation and optimization of structures for lossless ion manipulations for ion mobility spectrometry with time-of-flight mass spectrometry.Analytical chemistry · 2014Article
- Separation of protein conformers by differential ion mobility in hydrogen-rich gases.Analytical chemistry · 2013Article
- Carbohydrate structure characterization by tandem ion mobility mass spectrometry (IMMS)2.Analytical chemistry · 2013Article
- Protein analyses using differential ion mobility microchips with mass spectrometry.Analytical chemistry · 2012Article
- Conformational distribution of bradykinin [bk + 2 H]2+ revealed by cold ion spectroscopy coupled with FAIMS.Journal of the American Society for Mass Spectrometry · 2012Article
- High-resolution differential ion mobility separations using planar analyzers at elevated dispersion fields.Analytical chemistry · 2010Article
- High-resolution differential ion mobility separations using helium-rich gases.Analytical chemistry · 2010Article
- Differential ion mobility separations of peptides with resolving power exceeding 50.Analytical chemistry · 2010Article
- Ultrafast differential ion mobility spectrometry at extreme electric fields coupled to mass spectrometry.Analytical chemistry · 2009Article
- Ultrafast differential ion mobility spectrometry at extreme electric fields in multichannel microchips.Analytical chemistry · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent advancements in high-field asymmetric waveform ion mobility spectrometry (FAIMS) have led to significant improvements in the application of this technology to the study of protein conformers. Compared with previous work, the maximum value of the separation voltage (i.e., the dispersion voltage) has increased, thereby enabling multiple, elongated conformers of individual charge states of bovine ubiquitin to be separated in the gas phase (e.g., four conformers of each of the +11 and +12 charge states were separated). The use of a carrier gas mixture of 40% nitrogen and 60% helium changed the separation selectivity compared with pure nitrogen and enhanced the signal intensity, especially for the +14 and +15 charge states (the latter was not detected in a nitrogen carrier gas). Conformer cross sections were determined using the FAIMS/energy-loss method and found to be similar within a given charge state. The cross sections for conformers of charge states +13, + 14, and +15 plateau at about 2000 A2 suggesting that the structure of bovine ubiquitin is essentially unfolded after the addition of the 13th proton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.