ArticleNucleic acids research2001
Real-time observation of a single DNA digestion by lambda exonuclease under a fluorescence microscope field.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 75 citations in OpenAlex.
- 'Gel-Stacks' gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study.BioTechniques · 2024Article
- High-Throughput Exonuclease Assay Based on the Fluorescent Base Analogue 2-Aminopurine.ACS omega · 2023Article
- Rapid single-molecule characterisation of enzymes involved in nucleic-acid metabolism.Nucleic acids research · 2023Article
- DNA Manipulation and Single-Molecule Imaging.Molecules (Basel, Switzerland) · 2021Review
- Half a century of bacteriophage lambda recombinase: In vitro studies of lambda exonuclease and Red-beta annealase.IUBMB life · 2020Review
- Noncanonical substrate preference of lambda exonuclease for 5'-nonphosphate-ended dsDNA and a mismatch-induced acceleration effect on the enzymatic reaction.Nucleic acids research · 2018Article
- Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging.Beilstein journal of nanotechnology · 2017Article
- Genome modifications and cloning using a conjugally transferable recombineering system.Biotechnology reports (Amsterdam, Netherlands) · 2015Article
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Immobilization of lambda exonuclease onto polymer micropillar arrays for the solid-phase digestion of dsDNAs.Analytical chemistry · 2014Article
- Searching for electrical properties, phenomena and mechanisms in the construction and function of chromosomes.Computational and structural biotechnology journal · 2013Article
- Structural and functional insight into the mechanism of an alkaline exonuclease from Laribacter hongkongensis.Nucleic acids research · 2011Article
- Lambda exonuclease digestion of CGG trinucleotide repeats.European biophysics journal : EBJ · 2010Article
- Enzyme-modulated DNA translocation through a nanopore.Journal of the American Chemical Society · 2009Article
- Enzymatic digestion of single DNA molecules anchored on nanogold-modified surfaces.Nanoscale research letters · 2009Article
- The effect of physical form of DNA on exonucleaseIII activity revealed by single-molecule observations.Journal of fluorescence · 2009Article
- Novel, monomeric cyanine dyes as reporters for DNA helicase activity.Journal of fluorescence · 2007Article
- Detection of single DNA molecules by multicolor quantum-dot end-labeling.Nucleic acids research · 2005Article
- Single molecule fluorescence imaging and its application to the study of DNA condensation.Journal of fluorescence · 2004Article
- Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level.Nucleic acids research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fluorescence microscopy technique has been developed to visualize the behavior of individual DNA and protein molecules. Real-time direct observation of a single DNA molecule can be used to investigate the dynamics of DNA-protein interactions, such as the DNA digestion reaction by lambda exonuclease. In conventional methods it is impossible to analyze the dynamics of an individual lambda exonuclease molecule on a DNA because they can only observe the average behavior of a number of exonuclease molecules. Observation of a single molecule, on the other hand, can reveal processivity and binding rate of an individual exonuclease molecule. To evaluate the dynamics of lambda exonuclease, a stained lambda DNA molecule with one biotinylated terminal was fixed on an avidin-coated coverslip and straightened using a d.c. electric field. Microscopic observation of digestion of a straightened DNA molecule by lambda exonuclease revealed that the DNA digestion rate was approximately 1000 bases/s and also demonstrated high processivity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.