ArticleAnnals of the rheumatic diseases2001
Performance of two ELISAs for antifilaggrin autoantibodies, using either affinity purified or deiminated recombinant human filaggrin, in the diagnosis of rheumatoid arthritis.
Article in Annals of the rheumatic diseases, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Disordered Antigens and Epitope Overlap Between Anti-Citrullinated Protein Antibodies and Rheumatoid Factor in Rheumatoid Arthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
- Anti-citrullinated peptide antibodies and their value for predicting responses to biologic agents: a review.Rheumatology international · 2016Review
- Autoantigens as Partners in Initiation and Propagation of Autoimmune Rheumatic Diseases.Annual review of immunology · 2016Review
- In vitro eradication of citrullinated protein specific B-lymphocytes of rheumatoid arthritis patients by targeted bifunctional nanoparticles.Arthritis research & therapy · 2016Article
- Autoantibodies to posttranslational modifications in rheumatoid arthritis.Mediators of inflammation · 2014Review
- Anti-citrullinated peptide antibody assays and their role in the diagnosis of rheumatoid arthritis.Arthritis and rheumatism · 2009Review
- Peptide-coated nanotube-based biosensor for the detection of disease-specific autoantibodies in human serum.Biosensors & bioelectronics · 2008Article
- Diagnostic accuracy of immunoassays for the detection of antibodies to citrullinated proteins.Clinical reviews in allergy & immunology · 2008Review
- Prevalence and clinical significance of antibodies to citrullinated fibrinogen (ACF) in Chinese patients with rheumatoid arthritis.Clinical rheumatology · 2007Article
- Inhibitors and inactivators of protein arginine deiminase 4: functional and structural characterization.Biochemistry · 2006Article
- Diagnostic value of anti-human citrullinated fibrinogen ELISA and comparison with four other anti-citrullinated protein assays.Arthritis research & therapy · 2006Article
- Antibodies to citrullinated human fibrinogen (ACF) have diagnostic and prognostic value in early arthritis.Annals of the rheumatic diseases · 2005Article
- Diagnostic performances of anti-cyclic citrullinated peptides antibody and antifilaggrin antibody in Korean patients with rheumatoid arthritis.Journal of Korean medical science · 2005Article
- IgG subclass distribution of the rheumatoid arthritis-specific autoantibodies to citrullinated fibrin.Clinical and experimental immunology · 2005Article
- Rheumatoid factor and anticitrullinated protein antibodies in rheumatoid arthritis: diagnostic value, associations with radiological progression rate, and extra-articular manifestations.Annals of the rheumatic diseases · 2004Article
- Autoantibodies recognizing citrullinated rat filaggrin in an ELISA using citrullinated and non-citrullinated recombinant proteins as antigens are highly diagnostic for rheumatoid arthritis.Clinical and experimental immunology · 2004Article
- Rheumatoid arthritis specific anti-Sa antibodies target citrullinated vimentin.Arthritis research & therapy · 2004Article
- Autoantibody systems in rheumatoid arthritis: specificity, sensitivity and diagnostic value.Arthritis research · 2002Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo develop a standardisable enzyme linked immunosorbent assay (ELISA), using human filaggrin, for detection of antifilaggrin autoantibodies in rheumatoid arthritis (RA). To compare the diagnostic performance of the ELISA with those of reference tests: "antikeratin antibodies" ("AKA"), and antibodies to human epidermis filaggrin detected by immunoblotting (AhFA-IB).
methodsTwo ELISAs were developed using either affinity purified neutral-acidic human epidermis filaggrin (AhFA-ELISA-pur) or a recombinant human filaggrin deiminated in vitro (AhFA-ELISA-rec) as immunosorbent. Antifilaggrin autoantibodies were assayed in 714 serum samples from patients with well characterised rheumatic diseases, including 241 RA and 473 other rheumatic diseases, using the two ELISAs. "AKA" and AhFA-IB tests were carried out in the same series of patients. The diagnostic performance of the four tests was compared and their relationships analysed.
resultsThe titres of "AKA", AhFA-IB, and the AhFA-ELISAs correlated strongly with each other. The diagnostic sensitivity of the AhFA-ELISA-rec, which was better than that of AhFA-ELISA-pur, was 0.52 for a specificity of 0.95. This performance was similar to those of "AKA" or AhFA-IB. However, combining AhFA-ELISA-rec with AhFA-IB led to a diagnostic sensitivity of 0.55 for a specificity of 0.99.
conclusionA simple and easily standardisable ELISA for detection of antifilaggrin autoantibodies was developed and validated on a large series of patients using a citrullinated recombinant human filaggrin. The diagnostic performance of the test was similar to that of the "AKA" and AhFA-IB. Nevertheless, combining the AhFA-ELISA-rec with one of the other tests clearly enhanced the performance.
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Identifiers
11502616PMC1753817W2109786162What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.