ArticleFolia microbiologica2001
Development of competitive PCR for detection of Butyrivibrio fibrisolvens in the rumen.
Article in Folia microbiologica, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 10 citations in OpenAlex.
- Effect of fatty acids on growth of conjugated-linoleic-acids-producing bacteria in rumen.Folia microbiologica · 2006Article
- Diet-dependent shifts in ruminal butyrate-producing bacteria.Folia microbiologica · 2006Article
- Is Pseudobutyrivibrio xylanivorans strain Mz5T suitable as a probiotic? An in vitro study.Folia microbiologica · 2003Article
- Phenotypic and genetic data supporting reclassification of Butyrivibrio fibrisolvens isolates.Folia microbiologica · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Competitive PCR method was developed for the detection and enumeration of Butyrivibrio fibrisolvens. Sequences of 16S rDNA were obtained from our isolates (serving as a source of data for primer design) and were distinguished into nine different groups of butyrivibria. Specific primers for two distinct groups were designed with the help of BioEdit program. These primers were tested with DNA of 20 strains of ruminal B. fibrisolvens isolates. Annealing temperature 58 degrees C showed a little specificity but a better selectivity was found after raising it up to 65 degrees C. A group 1 competitive fragment of 16S rDNA of different length was constructed using restriction cutting with MspI followed by ligation; the size of the resulting fragment was cut down by 75 bp. The fragment worked in the presence of the original 16S rDNA fragment of B. fibrisolvens JK 609.
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