ArticlePlant physiology2001
Sequence architecture downstream of the initiator codon enhances gene expression and protein stability in plants.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 72 citations in OpenAlex.
- Suppressing Tymovirus replication in plants using a variant of ubiquitin.PLoS pathogens · 2025Article
- Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements.ACS synthetic biology · 2022Article
- Rapid validation of transcriptional enhancers using agrobacterium-mediated transient assay.Plant methods · 2019Article
- Stable production of cyanophycinase in Nicotiana benthamiana and its functionality to hydrolyse cyanophycin in the murine intestine.Plant biotechnology journal · 2017Article
- N-Terminal Acetylation-Targeted N-End Rule Proteolytic System: The Ac/N-End Rule Pathway.Molecules and cells · 2016Review
- Enzyme replacement for GM1-gangliosidosis: Uptake, lysosomal activation, and cellular disease correction using a novel β-galactosidase:RTB lectin fusion.Molecular genetics and metabolism · 2016Article
- Anti-infective immunoadhesins from plants.Plant biotechnology journal · 2015Review
- Expression and subcellular targeting of human complement factor C5a in Nicotiana species.PloS one · 2012Article
- High-level transient expression of ER-targeted human interleukin 6 in Nicotiana benthamiana.PloS one · 2012Article
- Interplay between N-terminal methionine excision and FtsH protease is essential for normal chloroplast development and function in Arabidopsis.The Plant cell · 2011Article
- Follow the leader: preference for specific amino acids directly following the initial methionine in proteins of different organisms.Genomics, proteomics & bioinformatics · 2010Article
- High level expression of a functionally active cholera toxin B: rabies glycoprotein fusion protein in tobacco seeds.Plant cell reports · 2009Article
- A T9G mutation in the prototype TATA-box TCACTATATATAG determines nucleosome formation and synergy with upstream activator sequences in plant promoters.Plant physiology · 2009Article
- Effect of point mutations in translation initiation context on the expression of recombinant human alpha(1)-proteinase inhibitor in transgenic tomato plants.Plant cell reports · 2009Article
- Two Dot1 isoforms in Saccharomyces cerevisiae as a result of leaky scanning by the ribosome.Nucleic acids research · 2009Article
- Diversity of preferred nucleotide sequences around the translation initiation codon in eukaryote genomes.Nucleic acids research · 2008Article
- AUG_hairpin: prediction of a downstream secondary structure influencing the recognition of a translation start site.BMC bioinformatics · 2007Article
- C-terminal motif prediction in eukaryotic proteomes using comparative genomics and statistical over-representation across protein families.BMC genomics · 2007Article
- The TATA-box sequence in the basal promoter contributes to determining light-dependent gene expression in plants.Plant physiology · 2006Article
- High level expression of surface glycoprotein of rabies virus in tobacco leaves and its immunoprotective activity in mice.Journal of biotechnology · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleotide positions conserved on the 3' side of the initiator codon ATG and the corresponding N-terminal amino acid residues in a number of highly abundant plant proteins were identified by computational analysis of a dataset of highly expressed plant genes. The reporter genes uidA and gfp were modified to introduce these features. Insertion of GCT TCC TCC after the initiator codon ATG augmented expression for both the reporter genes. The insertion of each successive codon improved the expression of beta-glucuronidase (GUS) in an incremental fashion in transient transformation of tobacco (Nicotiana tabacum) leaves. The insertion of alanine-serine (Ser)-Ser resulted in about a 2-fold increase in the stability of GUS. However, this did not account for the 30- to 40-fold increase in GUS activity between the constructs coding for methionine-alanine-Ser-Ser-GUS and the native enzyme. Substitution of the codon for Ser at the third amino acid residue with synonymous codons reduced GUS expression. The results suggest a role for the conserved nucleotides in the +4 to +11 region in augmenting posttranscriptional events in the expression of genes in plants.
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Registered trials
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