ArticlePlant physiology2001
Characterization of rice anthranilate synthase alpha-subunit genes OASA1 and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASA1.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 155 citations in OpenAlex.
- OsASA2 Loss-of-Function Confers Broad-Spectrum Disease Resistance and Triggers Cell Death by Regulating Anthranilate Synthase Activity in Rice.Molecular plant pathology · 2026Article
- OASA1D-Mediated Tryptophan Enrichment Improves Redox and Ionic Homeostasis Under Salt Stress in Rice.International journal of molecular sciences · 2026Article
- Decreased Expression of a Phosphoribosylanthranilate Transferase-Encoding Gene,International journal of molecular sciences · 2025Article
- Anthranilate at the interface of tryptophan and specialized metabolite biosynthesis.Frontiers in plant science · 2025Review
- Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Potassium transporter OsHAK17 may contribute to saline-alkaline tolerant mechanisms in rice (Oryza sativa).Journal of plant research · 2024Article
- Insight into de-regulation of amino acid feedback inhibition: a focus on structure analysis method.Microbial cell factories · 2023Review
- Genetically Modified Rice Is Associated with Hunger, Health, and Climate Resilience.Foods (Basel, Switzerland) · 2023Review
- Application of metabolic engineering to enhance the content of alkaloids in medicinal plants.Metabolic engineering communications · 2022Review
- Mining of Potential Gene Resources for Breeding Nutritionally Improved Maize.Plants (Basel, Switzerland) · 2022Review
- Growth-Defense Trade-Offs Induced by Long-term Overgrazing Could Act as a Stress Memory.Frontiers in plant science · 2022Article
- ThePlant signaling & behavior · 2021Article
- Jasmonates, Ethylene and Brassinosteroids Control Adventitious and Lateral Rooting as Stress Avoidance Responses to Heavy Metals and Metalloids.Biomolecules · 2021Review
- Homeobox transcription factor OsZHD2 promotes root meristem activity in rice by inducing ethylene biosynthesis.Journal of experimental botany · 2020Article
- Strategies for the production of biochemicals in bioenergy crops.Biotechnology for biofuels · 2020Review
- Nitric Oxide Cooperates With Auxin to Mitigate the Alterations in the Root System Caused by Cadmium and Arsenic.Frontiers in plant science · 2020Article
- Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice.PloS one · 2019Article
- Construction of a Quantitative Acetylomic Tissue Atlas in Rice (Molecules (Basel, Switzerland) · 2018Article
- Article
- Cloning and characterization of indole synthase (INS) and a putative tryptophan synthase α-subunit (TSA) genes from Polygonum tinctorium.Plant cell reports · 2016Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anthranilate synthase (AS) is a key enzyme in the synthesis of tryptophan (Trp), indole-3-acetic acid, and indole alkaloids. Two genes, OASA1 and OASA2, encoding AS alpha-subunits were isolated from a monocotyledonous plant, rice (Oryza sativa cv Nipponbare), and were characterized. A phylogenetic tree of AS alpha-subunits from various species revealed a close evolutionary relationship among OASA1 and Arabidopsis ASA2, Ruta graveolens AS alpha 2, and tobacco ASA2, whereas OASA2, Arabidopsis ASA1, and R. graveolens AS alpha 1 were more distantly related. OASA1 is expressed in all tissues tested, but the amount of its mRNA was greater in panicles than in leaves and roots. The abundance of OASA2 transcripts is similar among tissues and greater than that of OASA1 transcripts; furthermore, OASA2 expression was induced by a chitin heptamer, a potent elicitor, suggesting that OASA2 participates in secondary metabolism. Expression of wild-type OASA1 or OASA2 transgenes did not affect the Trp content of rice calli or plants. However, transformed calli and plants expressing a mutated OASA1 gene, OASA1(D323N), that encodes a protein in which aspartate-323 is replaced with asparagine manifested up to 180- and 35-fold increases, respectively, in Trp accumulation. These transgenic calli and plants were resistant to 300 microM 5-methyl-Trp, and AS activity of the calli showed a markedly reduced sensitivity to Trp. These results show that OASA1 is important in the regulation of free Trp concentration, and that mutation of OASA1 to render the encoded protein insensitive to feedback inhibition results in accumulation of Trp at high levels. The OASA1(D323N) transgene may prove useful for the generation of crops with an increased Trp content.
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